Investigation on phytochemical screening analytical microbiological and pharmacological activity of different ficus species

Abstract

The research work highlights the Investigation on phytochemical screening, analytical, microbiological and pharmacological activity of different Ficus species . The selected Ficus benghalensis aerial roots and Ficus religiosa leaves were collected from in and around rajampet in the month of Sep 09 and authenticated. Plant materials were dried, powdered and extracted by continuous hot percolation method using various solvents in the order of increasing polarity. The constituent present in the extrtacts were screened by phyto chemical test. Ficus benghalensis aerial roots extracts shows the presence of triterpenoids, alkaloids, flavonoids and Ficus religiosa leaves extracts shows the presence of terpenoids, flavonoids, alkaloids, phenolic compounds and tannins. The flavonoid rich extracts were evaluated for microbiological activity. The methanolic and ethylacetate extracts of Ficus benghalensis aerial roots (MEFB and EEFB) and the methanolic and ethylacetate extracts of Ficus religiosa leaves (MEFR and EEFR) were performed for antimicrobial activity respectively by cup plate method on Muller Hington agar medium against standard drugs (Tetracycline, Erythromycin). The MEFB shows active against P.vulgaris, S.typhi, S.epidermidis, S.griseus, and E.coli and EEFB was active against S.aureus, S.typhi, S.epidermidis, Lacto bacillus, C. diphtheriae, and E.coli. On other hand the MEFR shows active against S. aureus, S. typhi, S. griseus and E. coli and EEFR active against S. aureus, S. typhi, S. griseus, P. vulgaris and E. coli. The zone of inhibition of methanolic extracts shown very high inhibition when compare with ethyl acetate extracts. The minimum inhibitory concentrations (MIC) for methanolic extract of both species i.e MEFB, MEFR were found to be 50and#956;g/ml and 100 and#956;g/ ml respectively. Antifungal activity was done by diffusion plate method using Sabouraud dextrose agar (SDA) medium. Three different Candida albicans, Monilinia fruticola, Auricularia polytricha) fungal organisms were tested against the methanolic extract of Ficus.