Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/9030
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dc.coverage.spatialbotanyen_US
dc.date.accessioned2013-05-21T11:21:32Z-
dc.date.available2013-05-21T11:21:32Z-
dc.date.issued2013-05-21-
dc.identifier.urihttp://hdl.handle.net/10603/9030-
dc.description.abstractAdventitious root (AR) formation from the meristematic activity in the predetermined interfascicular region of the stem, has been under investigation in the past, largely for the associated anatomical and physiological aspects. Recently, however, interesting observations on the biochemical and molecular events accompanying the three phases of AR formation (induction, initiation and extension) have also been put forward. On line with these investigations, present work focuses on auxin modulated AR formation in hypocotyl segments derived from sunflower seedlings. Major attention has been paid to localize differential nitric oxide accumulation at/around the site of AR formation and during different phases of AR development. Based on these investigations, a model has been proposed about auxin-nitric oxide (NO) interaction during specific stages of AR formation. Going deeper into the mechanism of NO action during AR formation, tyrosine nitration of proteins has been investigated as a possible signalling route accompanying the said response. Both spatial distribution in sections and Western blotting analysis of tyrosine nitrated proteins in tissue homogenates, have generated significant new information. Analysis of actin distribution by confocal laser scanning microscopic imaging has emphasized the significance of intact cytoskeleton for polarized distribution of PIN (auxin efflux) proteins, and their modulation by nitric oxide scavenger and auxin efflux blocker in a similar manner. A bundling/debundling of actin network seems to regulate directional auxin movement by regulating the movement of PIN carrying vesicles through the process of endomembrane recycling. Two-dimensional electrophoretic separation of polypeptides has lead to interesting identification of polypeptides unique to specific stages of AR formation. In addition to these investigations, attention has also been paid to observe a possible correlation of this auxin-regulated developmental process, with mechanisms for scavenging reactive oxygen species.en_US
dc.format.extent106p.en_US
dc.languageEnglishen_US
dc.relation-en_US
dc.rightsuniversityen_US
dc.titleModulation of adventitious rooting in sunflower hypocotyls by nitric oxide, auxin and other related biomoleculesen_US
dc.title.alternative-en_US
dc.creator.researcherYadav, Sunitaen_US
dc.subject.keywordsunfloweren_US
dc.subject.keywordBotanyen_US
dc.description.noteReferences p.88-106en_US
dc.contributor.guideBhatla, S Cen_US
dc.publisher.placeNew Delhien_US
dc.publisher.universityUniversity of Delhien_US
dc.publisher.institutionDept. of Botanyen_US
dc.date.registeredn.d.en_US
dc.date.completed2012en_US
dc.date.awardedn.d.en_US
dc.format.dimensions-en_US
dc.format.accompanyingmaterialNoneen_US
dc.type.degreePh.D.en_US
dc.source.inflibnetINFLIBNETen_US
Appears in Departments:Dept. of Botany

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01_title.pdfAttached File45.03 kBAdobe PDFView/Open
02_certificate.pdf30.59 kBAdobe PDFView/Open
03_preface.pdf22.61 kBAdobe PDFView/Open
04_acknowledgements.pdf33.67 kBAdobe PDFView/Open
05_contents.pdf55.6 kBAdobe PDFView/Open
06_chapter 1.pdf93.49 kBAdobe PDFView/Open
07_chapter 2.pdf825.74 kBAdobe PDFView/Open
08_chapter 3.pdf419.66 kBAdobe PDFView/Open
09_chapter 4.pdf604.65 kBAdobe PDFView/Open
10_chapter 5.pdf123.45 kBAdobe PDFView/Open
11_references.pdf423.67 kBAdobe PDFView/Open
12_research publications.pdf1.97 MBAdobe PDFView/Open
13_plates.pdf239.4 MBAdobe PDFView/Open
14_abstract.pdf20.63 kBAdobe PDFView/Open


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