Computational bioactivity analysis of major withanolides and their bioactivity analysis in in vitro cultures of Withania species

Abstract

Major objectives The present study was deviced with the following objectives To develop a reproducible protocol for in vitro shoot multiplication, adventitious root induction and its mass cultivation of W Coagulans To identify the potential withanolide as anticancer and antiinflammatory agent using in silico tools To validate the results obtained from in silico analysis using cell cultures newlineHypothesis The present study was designed to test the following hypothesis Null hypothesis H The crude extracts of withania tissues grown in vitro and the withanolides do not possess significant bioactivity in them Alternate hypothesis HA The crude extracts of withania tissues grown in vitro and the withanolides possess significant bioactivity newline