Immunostimulant action of levamisole in nanoparticles

Abstract

The objective of the preset research work was to maintain immunity by targeting newlineimmunostimulant drug Levamisole to Peyer s patch. Lack of immunity can cause infectious newlinediseases; cancer; AIDS; TB, etc. in which lymph node plays very important role. Nanoparticles newlinehaving size less than 150 nm can be absorbed by Peyer s patch and thus reaches to lymphatic newlinesystem. Thus the objective was fulfilled by formation of Levamisole nanoparticles, which was newlineable to target Peyer s patch. Levamisole Hydrochloride is hydrophilic in nature, and so the newlinepopular nanoprecipitation method is not much useful. The nanoparticles were prepared using newlineionic gelation method in which nanoparticles are generated due to interaction between/ within newlinepositively charged Chitosan and negatively charged TPP ion of sodium tri poly phosphate newline(STPP). Many factors like Chitosan concentration, negative charge generating agent, pH of newlineChitosan solution, pH of drug solution, pH adjusting agent, Chitosan/negative charge generating newlineagent ratio, drug concentration are having influence on particle size, surface charge, release newlinecharacteristics and percentage drug entrapment efficiency. Preliminary parameters were selected newlinelike 10% ammonia solution as pH adjusting agent; STPP as negative charge generating agent; 2 newlinemg/ml Chitosan concentration and Chitosan solution pH 4.0. A design of experiment was newlinegenerated having three factors like Chitosan/STPP ratio; pH of drug dissolved STPP solution and newlineLevamisole concentration with three level having three responses like particle size (nm); zeta potential (mv) and % entrapment. A regression analysis was carried out generating mathematical newlineequations which have shown good correlation ship between factors and responses. The optimized formulation was selected on bases of small average particle size, high zeta potential and high % entrapment value. Scale up of optimized formulation was done and was further characterized.