Isolation and Characterization of Antidiabetic and Antioxidant Principles from Saraca indica and Polyalthia longifolia bark

Abstract

Diabetes mellitus is a heterogenous disorder characterized by derangement of carbohydrate protein and lipid metabolism The present study aimed at investigation of the pharmacological potential of the barks of two medicinal plants Saraca indica and Polyalthia longifolia in the management of diabetes mellitus In vitro antidiabetic and antioxidant potential were assessed by studying the inhibition of alpha amylase and alpha glucosidase activities and free radical scavenging assays by various solvent extracts of the barks The ethanolic extract of both the barks proved to be potent from in vitro studies and exhibited moderate cytotoxicity in Vero cell lines and L6 celllines Results of the in vivo antidiabetic and antioxidant potential of the ethanolic extracts of the barks of Saraca indica and newlinePolyalthia longifolia revealed that the diabetic rats treated with SIE and PLE showedsignificant improvement in the level of blood glucose lipid profile renal function parameters and activities of enzyme markers SGOT SGPT ALP LDH in the serum of experimental rats compared to NIASTZ induced diabetic rats The activities of hepatic enzymic antioxidants catalase superoxide dismutase glutathione peroxidase glutathione reductase and the level newlineof nonenzymic antioxidants Vitamin C Vitamin E and reduced glutathione were found to significantly increase in the extracts treated diabetic rats Hepatic carbohydrate metabolizing newlineenzymes glucokinase glucose6phosphatase glucose6phosphate dehydrogenase succinate dehydrogenase and malate dehydrogenase were found to significantly increase in newlinethe extracts treated diabetic rats Histopathological analysis revealed protection to damage in the live, pancreas and kidney of SIE PLE treated diabetic rats Immunohistochemical analysis revealed betacell regeneration of the pancreas in the extracts treated diabetic rats Major objectives Analysis of phytochemicals and inorganic elements in the barks of Saraca indica and Polyalthia longifolia newlineAssessment of alphaamylase alphaglucosidase inhibitory activities