Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/6127
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dc.coverage.spatialCrop Physiologyen_US
dc.date.accessioned2013-01-08T07:14:20Z-
dc.date.available2013-01-08T07:14:20Z-
dc.date.issued2013-01-08-
dc.identifier.urihttp://hdl.handle.net/10603/6127-
dc.description.abstractRice is widely cultivated under irrigated condition and its water requirement is very newlinehigh. Due to dwindling water resources, to save irrigation water growing rice under newlinesemi-irrigated aerobic condition has phenomenal relevance. In this ecosystem decreased water availability, high VPD affects crop growth and productivity. From this context it is important to improve adaptation of rice under aerobic condition by improving water relations and cellular level tolerance mechanisms. Stress adaptation at cellular level involves activation of stress responsive genes which are regulated by transcription factors. Hence, under stress, co-ordinated expression of multiple transcription factors is crucial to increase cellular level tolerance. As a first step a multigene construct coexpressing GUS, AtbHLH17 and AtWRKY28 was developed using modified multisite gateway technology and developed transgenics in Arabidopsis. These transgenics showed improved tolerance to diverse stresses like drought, salinity and oxidative stress by upregulation of large number of downstream genes. Further, three stress responsive TFs from different families i.e EcNAC1, EcMYC57, EcbZIP60 were cloned from fingermillet and validated their relevance in stress tolerance in tobacco. Tobacco transgenics co-expressing all the three TFs showed superior phenotype under stress compared to single TFs expressing transgenics signifying the importance of co newlineexpression of relevant stress specific TFs. To improve adaptation of rice under semiirrigated aerobic conditions rice transgenics were developed co-expressing EcNAC1, EcMYC57 and EcbZIP60 in the background of genotype having superior water relations. Desirable transformants were identified based on dessication response. Molecular characterization of transformants showed the integration and expression of all three genes. The transgenics showed improved tolerance to salinity and oxidative stress.en_US
dc.format.extent187p.en_US
dc.languageEnglishen_US
dc.relation--en_US
dc.rightsuniversityen_US
dc.titleDevelopment of multiple gene construct with regulatory genes and their functional validationen_US
dc.creator.researcherBabitha K Cen_US
dc.subject.keywordCrop Physiologyen_US
dc.description.noteSummary p. 140-146, References p. 147-187, Appendix includeden_US
dc.contributor.guideUdayakumar Men_US
dc.publisher.placeBangaloreen_US
dc.publisher.universityUniversity of Agricultural Sciences, Bangaloreen_US
dc.publisher.institutionDepartment of Crop Physiologyen_US
dc.date.registeredn.d.en_US
dc.date.completedNovember, 2012en_US
dc.date.awarded2012en_US
dc.format.dimensions--en_US
dc.format.accompanyingmaterialNoneen_US
dc.type.degreePh.D.en_US
dc.source.inflibnetINFLIBNETen_US
Appears in Departments:09Crop Physiology

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01_title.pdfAttached File78 kBAdobe PDFView/Open
02_certificate.pdf48.96 kBAdobe PDFView/Open
03_acknowledgement.pdf84.65 kBAdobe PDFView/Open
04_abstract.pdf141.82 kBAdobe PDFView/Open
05_contents.pdf56.03 kBAdobe PDFView/Open
06_list of table figures & plates.pdf122.68 kBAdobe PDFView/Open
07_chapter 1.pdf89.17 kBAdobe PDFView/Open
08_chapter 2.pdf317.55 kBAdobe PDFView/Open
09_chapter 3.pdf250.3 kBAdobe PDFView/Open
10_chapter 4.pdf16.73 MBAdobe PDFView/Open
11_chapter 5.pdf242.89 kBAdobe PDFView/Open
12_chapter 6.pdf108.2 kBAdobe PDFView/Open
13_references.pdf342.28 kBAdobe PDFView/Open
14_appendix.pdf782.21 kBAdobe PDFView/Open


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