Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/612395
Title: Correlation of Gut Microbial Diversity with Biomarkers of Inflammation and Gut Permeability and Its Stability in Patients with Crohns Disease
Researcher: Amit Kumar Dutta
Guide(s): Joseph A J and Uday George Zachariah
Keywords: Biomarkers
Crohn s Disease
Gut Microbial Diversity
Gut Permeability
Inflammation
University: The Tamil Nadu Dr. M.G.R. Medical University
Completed Date: 2022
Abstract: Microbial diversity correlates inversely with CRP (Marker of inflammation) but not Fecal Calprotectin. Microbial diversity correlates inversely with sCD14 (Marker of gut permeability) and zonulin (Marker of gut permeability) but not alpha1 antitrypsin. Gut permeability is abnormal in majority of patients with active as well as stable CD based on lactulose mannitol absorption test. The diversity and composition of bacterial community is stable in patients with stable CD. Gut microbial diversity is different between patients with CD and healthy controls. Gut microbial diversity is different between patients with active CD and stable CD. Random forest analysis identified certain bacterial taxonomic predictors of disease status. The genera Tanerellaceae, Peptosterptococcaceae, Fusobacteriaceae, Morganellaceae and Pseudomonadeceae were predictors of active Crohn s disease. There are significant differences in some of the predicted metagenomic function of gut bacteria in active CD compared to healthy controls. In conclusion, our work has shown the dynamics of gut bacterial diversity with biomarkers of inflammation and gut permeability. It also shows the stability of gut bacterial composition over a period of time in patients with stable disease. Impact of the study: This research work has shown the inverse correlation of biomarker of inflammation (CRP) with bacterial alpha diversity. This observation suggests the link of gut bacteria with disease activity. Among biomarkers of inflammation, sCD14 had inverse correlation with bacterial diversity and appears to be a potentially useful biomarker of disease for clinical application. Perhaps a select group of bacteria may be better a predictor of inflammatory activity and gut bacterial dysfunction and future studies may be designed to explore this. A key observation in our work was the temporal stability of gut microbiota in patients with stable CD. This was apparent on alpha diversity, beta diversity and actual taxonomic constituents.
Pagination: 175
URI: http://hdl.handle.net/10603/612395
Appears in Departments:Department of Medical

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02_prelim pages.pdf1.68 MBAdobe PDFView/Open
03_content.pdf155.71 kBAdobe PDFView/Open
05_chapter 1.pdf174.55 kBAdobe PDFView/Open
06_chapter 2.pdf169.09 kBAdobe PDFView/Open
07_chapter 3.pdf712.15 kBAdobe PDFView/Open
08_chapter 4.pdf478.62 kBAdobe PDFView/Open
09_chapter 5.pdf5.1 MBAdobe PDFView/Open
10_annexures.pdf805.26 kBAdobe PDFView/Open
10_chapter 6.pdf224.77 kBAdobe PDFView/Open
80_recommendation.pdf401.72 kBAdobe PDFView/Open
90_plagiarism_report.pdf169.04 kBAdobe PDFView/Open
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