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http://hdl.handle.net/10603/612395
Title: | Correlation of Gut Microbial Diversity with Biomarkers of Inflammation and Gut Permeability and Its Stability in Patients with Crohns Disease |
Researcher: | Amit Kumar Dutta |
Guide(s): | Joseph A J and Uday George Zachariah |
Keywords: | Biomarkers Crohn s Disease Gut Microbial Diversity Gut Permeability Inflammation |
University: | The Tamil Nadu Dr. M.G.R. Medical University |
Completed Date: | 2022 |
Abstract: | Microbial diversity correlates inversely with CRP (Marker of inflammation) but not Fecal Calprotectin. Microbial diversity correlates inversely with sCD14 (Marker of gut permeability) and zonulin (Marker of gut permeability) but not alpha1 antitrypsin. Gut permeability is abnormal in majority of patients with active as well as stable CD based on lactulose mannitol absorption test. The diversity and composition of bacterial community is stable in patients with stable CD. Gut microbial diversity is different between patients with CD and healthy controls. Gut microbial diversity is different between patients with active CD and stable CD. Random forest analysis identified certain bacterial taxonomic predictors of disease status. The genera Tanerellaceae, Peptosterptococcaceae, Fusobacteriaceae, Morganellaceae and Pseudomonadeceae were predictors of active Crohn s disease. There are significant differences in some of the predicted metagenomic function of gut bacteria in active CD compared to healthy controls. In conclusion, our work has shown the dynamics of gut bacterial diversity with biomarkers of inflammation and gut permeability. It also shows the stability of gut bacterial composition over a period of time in patients with stable disease. Impact of the study: This research work has shown the inverse correlation of biomarker of inflammation (CRP) with bacterial alpha diversity. This observation suggests the link of gut bacteria with disease activity. Among biomarkers of inflammation, sCD14 had inverse correlation with bacterial diversity and appears to be a potentially useful biomarker of disease for clinical application. Perhaps a select group of bacteria may be better a predictor of inflammatory activity and gut bacterial dysfunction and future studies may be designed to explore this. A key observation in our work was the temporal stability of gut microbiota in patients with stable CD. This was apparent on alpha diversity, beta diversity and actual taxonomic constituents. |
Pagination: | 175 |
URI: | http://hdl.handle.net/10603/612395 |
Appears in Departments: | Department of Medical |
Files in This Item:
File | Description | Size | Format | |
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01_title.pdf | Attached File | 124.51 kB | Adobe PDF | View/Open |
02_prelim pages.pdf | 1.68 MB | Adobe PDF | View/Open | |
03_content.pdf | 155.71 kB | Adobe PDF | View/Open | |
05_chapter 1.pdf | 174.55 kB | Adobe PDF | View/Open | |
06_chapter 2.pdf | 169.09 kB | Adobe PDF | View/Open | |
07_chapter 3.pdf | 712.15 kB | Adobe PDF | View/Open | |
08_chapter 4.pdf | 478.62 kB | Adobe PDF | View/Open | |
09_chapter 5.pdf | 5.1 MB | Adobe PDF | View/Open | |
10_annexures.pdf | 805.26 kB | Adobe PDF | View/Open | |
10_chapter 6.pdf | 224.77 kB | Adobe PDF | View/Open | |
80_recommendation.pdf | 401.72 kB | Adobe PDF | View/Open | |
90_plagiarism_report.pdf | 169.04 kB | Adobe PDF | View/Open |
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