A Microbiological Study of Metallo and#914; Lactamase Producing Pseudomonas Aeruginosa and Its Antimicrobial Resistance Pattern Isolated from Various Clinical Specimens at A Tertiary Care Hospital Udaipur Rajasthan

Abstract

INTRODUCTION: newlinePseudomonas aeruginosa represents a great threat to public health newlineworldwide, due to its high ability to acquire resistance to different antibiotic newlineclasses. Carbapenems are effective against multidrug resistant (MDR) newlinePseudomonas aeruginosa, but their widespread use has resulted in the emergence newlineof carbapenem-resistant strains, which is considered a major global concern. This newlinestudy aimed to determine the MBL producing Pseudomonas aeruginosa and its newlineantimicrobial resistance pattern isolated from different clinical samples. newlineAIMS: newlineand#61623; Isolation and identification of Pseudomonas species newlineand#61623; To find out prevalence of Pseudomonas aeruginosa from various clinical newlinesamples. newlineand#61623; To study antimicrobial susceptibility pattern of Pseudomonas aeruginosa newlineisolated from various clinical samples for detection of antimicrobial newlineresistance strain. newlineand#61623; To detect Metallo beta lactamase in Pseudomonas species isolated from newlinevarious clinical samples newlineand#61623; To compare the antimicrobial resistance pattern of Pseudomonas newlineaeruginosa isolated from various clinical samples from indoor and outdoor newlinepatients attending hospital. newlineOBJECTIVES: newlinePrimary Objective newlineand#61623; Present study will help to know the prevalence of Pseudomonas newlineaeruginosa species from various clinical samples and their antimicrobial newlineresistant pattern. newlineSecondary Objective newlineand#61623; Detection and close monitoring of drug resistant strain will help in newlinemanagement of infections caused by Pseudomonas aeruginosa species and newlineformulation of antibiotic policy in hospital and appropriate infection newlinecontrol measures to reduce the incidence of antimicrobial drug resistance. newlineMATERIALS AND METHODS newlineDesign of the Study: newlineand#61623; This prospective study was carried out at the Department of Microbiology newlineat Pacific Medical College and Hospital in Udaipur, Rajasthan, from May newline2020 to December 2023. newlineand#61623; In the laboratory, 1000 samples were processed to identify Pseudomonas newlineaeruginosa species of which 300 were isolated and identified. newlineand#61623; The isolated Pseudomonas species were then processed for identification of newlinePseudomonas aerugino