Study of biomedical applications based on iron oxide nanoparticle nucleic acid conjugates

Abstract

newlineThis study explores the biomedical applications of iron oxide nanoparticle-nucleic acid conjugates, focusing on their potential in targeted biomedical functions like drug delivery, gene therapy, and diagnostic imaging. The introduction emphasizes the unique properties of iron oxide nanoparticles, including biocompatibility, magnetic responsiveness, and ease of surface functionalization, which make them suitable for advanced biomedical applications. Thesis emphasizes the synthesis and characterization of glycine-modified magnetic iron oxide nanoparticles (glycineatFe3O4) using various techniques such as Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Dynamic Light Scattering (DLS), revealing their potential for versatile biomedical applications (bacterial nucleic acid separation, DNA magnetofection, and viral pathogen detection). newlineIt investigates the adsorption and desorption of nucleic acids with glycineatFe3O4, providing insights into optimizing these processes for effective glycineatFe3O4 nucleic acid conjugate formation. Further, the thesis discusses the application of glycineatFe3O4 for DNA separation from Klebsiella pneumoniae, showcasing a novel electrochemical sensing approach that enables rapid and sensitive detection of nosocomial infections. newlineFurthermore, the thesis presents a study on magnetofection for gene (DNA) delivery in breast cancer cells using glycineatFe3O4 conjugated with fluorescent carbon quantum dots (glycineatFe3O4atQDs). The findings highlight successful intracellular delivery and reduced cytotoxicity, marking this method as a promising strategy for magnetofection. newlineLastly, thesis introduces a novel method for SARS-CoV-2 RNA detection using glycineatFe3O4, which demonstrates efficient binding and extraction of RNA, yielding results comparable to conventional methods. newlineOverall, this study underscores the transformative potential of iron oxide-nucleic acid conjugates in advancing personalized medicine and enhancing diagnostic capabilities in healthcare. newline