Evaluation of Siderophore and Acc 1 Aminocyclopropane 1 Carboxylic Acid Deaminase Producing Bacteria for the Management of Abiotic and Biotic Stresses

Abstract

The present study aimed to exploit siderophore and ACC deaminase ACCD producing bacteria to mitigage abiotic salinity, drought, and heavy metals and biotic Ralstonia solanacearum and Xanthomonas Oryzae pv. oryzae Xoo stresses and simultaneously enhance the essential nutrients N and P micronutrients co and fe relative water content RWC and ChlorophyII pigment. In this study, 250 bacteria ware isolated from 25 agricultural and non agricultural soils. Sreening revealed that 21.2 percentage of the isolates possessed both siderophore and ACCD activity. Based on multiple screening for stress tolerance plant growth promoting properties and plate assay in black gram resulted in the selection of four isolates. Furthermore, evaluation in pot studies revealed enhanced root and shoot length compared to the control in all stressed plants. Inoculated plants also enhanced the uptake of N and P, and chlorophyII content. The bacterial inoculation also enhanced siderophore and ACCD production and soil respiration activity in the stressed soils. Furthermore, two most potent siderophore producing B. subtilis CWTS 5 and B. albus CWTS 10 significantly inhibited both R. solanacearum and X oryzae pv. oryzae Xoo under in vitro and in Vivo conditions. FTIR, LC MS and whole genome sequencing of these strains revealed multiple anitmicrobials, secondary metabolites and siderophore componds. Therefore, the B. subtilis CWTS 5 and B albus CWTS 10 strains identified in this study can be formulated as bioinoculants to manage abiotic and biotic stresses. newline newline newline