Development Of Stability Indicating Assay Methods For Estimation Of Some New Drugs In Pharmaceutical Formulation

Abstract

The aim of this research work was develop simple, precise and rapid RP-HPLC Method of newlineanalysis of Sitagliptin and metformin, Vildagliptin and metformin, Saxagliptin and metformin, newlinecarisoprodol and ibuprofen and silymarin and ursodeoxycholic acid in tablet dosage form using newlineLC/MS Method. newline RP-HPLC method was developed for the estimation of Sitagliptin and metformin, newlineVildagliptin and metformin, Saxagliptin and metformin, carisoprodol and ibuprofen and newlinesilymarin and ursodeoxycholic acid in tablet dosage form with the help of HPLC approaches. newlineThe proposed methods were applied for the determination of drug in tablet dosage form. newlineDetermination of Sitagliptin and metformin, Vildagliptin and metformin, Saxagliptin and newlinemetformin, carisoprodol and ibuprofen and silymarin and ursodeoxycholic acid is equation newlinemethod. In this method concentration of each drug was obtained by using the absorptivity values newlinecalculated for drug wavelength 215 nm, 225 nm, 218 nm, 224 nm and 258 nm respectively and newlinesolving the equation. A rapid and reliable RP-HPLC method was developed and validated newlineestimation of Sitagliptin and metformin, Vildagliptin and metformin, Saxagliptin and metformin, newlinecarisoprodol and ibuprofen and silymarin and ursodeoxycholic acid in tablet dosage form. The newlineRP-HPLC method was performed C18-(100mm x 4.6 mm,)2.5 and#956;m particle size in gradient newlinemode, and the sample was analyzed using acetonitrile and pH buffer acetic acid (0.05% OPA newlinewith TEA) as a mobile phase for Sitagliptin and metformin 35:65 ml, 0.7 ml flow, By the newlineretention time for Sitagliptin and metformin, found 3.39 and 4.59 min respectively. for newlineVildagliptin and metformin 40 : 60 ml, 0.7 ml flow By the retention time for Vildagliptin and newlinemetformin found 3.39 and 4.59 min respectively. newline