Investigations of metallicnano particles in cancer therapeutics

Abstract

The main objective of the present thesis involves Investigations of Metallic nanoparticles in Cancer therapeutics . Metal nanoparticles were synthesized using the green method. In the green method, Leaf extracts were obtained from Litchi chinensis leaf. AgNPs and AuNPs were synthesized using the Litchi chinensis leaf extract. To understand their role in biogenesis, we have investigated the effect of various physicochemical parameters like metal ion concentration, leaf extract quantity, reaction time, temperature and pH effect. We have concluded the optimal quantity of 15 and#956;l of LCLE, 1 mM metal ion concentration, 25 °C reaction temperature, and 30 min for completion of the reaction for the formation of AgNPs and AuNPs. The post-synthesis stability of AgNPs was studied under different pH conditions. Secondly, the Citrate and PVP-capped silver and gold nanoparticles were synthesized. The bimetallic nanoparticles of silver and gold metals were also synthesized using the green method. Silver core gold nanoparticles and Gold core silver nanoparticles were synthesized using LCLE. The anticancer activity of silver, gold and bimetallic nanoparticles was studied against breast cancer cell lines (MCF-7). The formation of stable metal nanoparticles was confirmed using different characterization techniques. The effect of various synthetic strategies on cellular-related studies such as trypan blue exclusion for cell viability, cell morphological changes using microscopic analysis, cell cytotoxicity studies using MTT assays, and acridine and ethidium bromide staining for DNA damage studies were performed. Green synthesis-based nanoparticles have almost similar cytotoxicity as chemically synthesized nanoparticles. In the green method we use no hazardous chemicals to synthesize metal nanoparticles, whereas, in the chemical, we have used chemicals that may be harmful to healthy cells too. newline