Acute Sub acute Chronic Toxicological and Neuropharmacological Evaluation of Flowers of Nyctanthes arbor tristis Linn in Wistar Albino Rats

Abstract

AIM- The aim of the present investigation was to evaluate Neurological Investigation and Toxicity Evaluation of Ethanolic Extract of Flowers of Nyctanthes arbor-tristis Linn. by Different Models. MATERIAL and METHODS- The plant material i.e. flowers were dried under shade and subjected to coarse powder for extraction process. Accurately weighed quantity of flower powder of Nyctanthes arbor-tristis were extracted using petroleum ether for the removal of fats and then different solvents were used according to polarity charts. Qualitative chemical tests of different extracts were subjected to various chemical tests to detect various phytoconstituents. The animals were under observation for their behavioral changes if any, at 30 minutes intervals in the first one hour and at the hourly intervals for the next 4 hour for the following parameters. Locomotor activity is easily measured using actophotometer which operates on photoelectric cells connected with a counter. When a beam of light falling on the photocell is cut off by the animal a count is recorded and displayed digitally. The animals were discarded and replaced if they failed to do so. All the groups animals were placed on the rota rod and the fall off time from the rotating rod was noted after 60 minutes of test drug and standard administration. RESULTS- The preliminary phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds. Rats treated with ethanolic extract of Nyctanthes arbor-tristis and were submitted to general behavioural profile studies did not show any difference in their behaviors. The ethanolic extract of Nyctanthes arbor-tristis in a dose level of 200 mg/kg, and 400 mg/kg, p.o did not produce newlinexv newlinestatistically any significant reduction in locomotor activity as compared to the control animals receiving only the vehicle. There was statistically significant decrease in the time of falls within 3 minutes after the treatment with ethanolic extract of Nyctanthes arbor-tristis at dose level of 200 mg/kg and 400 mg/kg, p.o and result reveals that Nyctanthes arbor-tristis has no muscle relaxant property newline