Formulation Characterization and Evaluation of Nanoparticle Mediated Targeted Nose to Brain Drug Delivery Systems

Abstract

The primary objective of the current research is to formulate, characterize, and evaluate nanoparticle-mediated targeted nose-to-brain drug delivery systems for anticancer drugs, to increase their availability in the central nervous system (CNS). newlineMethods: newlinePEG-PLGA nanoparticles, synthesized via the emulsification solvent evaporation technique, involve the integration of PLGA, a biodegradable polymer, and PEG, a hydrophilic polymer. These nanoparticles enhance drug delivery and efficacy in the body. The preparation begins with dissolving PEG-PLGA in acetone ((CH3)2CO) to form a solution. This solution is then mixed with an aqueous solution containing polyvinyl alcohol (PVA) to create an emulsion. Sonication of the emulsion results in the formation of smaller droplets. Subsequently, the acetone is evaporated, leading to the formation of PEG-PLGA nanoparticles. Finally, the nanoparticles are collected and washed to remove any residual solvents and impurities. newlineThe prepared nanoparticles were evaluated based on their particle size, Transmission Electron Microscopy (TEM) analysis, zeta potential, Hemagglutination assay, loading capacity, and entrapment efficiency (%EE). newline