analysis of some anti diabetic agents in bulk and their synthetic mixture by chromatographic methods

Abstract

RP-HPLC: The degradation study revealed that MET, SAXA, and DAPA followed first-order kinetics in all stress conditions as all the Log concentration vs. time graphs were linear with a regression coefficient near 1. With the increase in 20-degree temperature, the reaction rate increased by almost two folds, and the rate constant increased. As per the Arrhenius equation, ln k is inversely proportional to temperature., which was demonstrated experimentally that the value of the constant increased with an increase in temperature. All three drugs were more liable to oxidative conditions while showing good photolytic study stability. HPTLC: MET showed slower degradation than DAPA and SAXA in both acidic and basic conditions. The proposed method is precise, and the percentage relative standard deviation was between 0.5 and 2.0. The observed percentage recoveries were between 96.7 and 99.54 for all three compounds. The results from the degradation study stipulated that on exposure to various stressors, namely acid, alkali, oxidative, and UV light, the MET, DAPA, and SAXA. t-test was applied to compare the results of % Recovery for pure drugs. The results of and t-test revealed that calculated T values were less than the tabulated values, stating no significant difference between the two methods, indicating that the proposed RP-HPLC and LC-MS/MS appear to be equally suiTable for routine determination of ALO and PIO simultaneously in formulations. newline