Molecular Characterization of Drug Resistant Klebsiella Pneumoniae Isolates from the Clinical Specimens of the Patients Attending Tertiary Care Hospital

Abstract

ABSTRACT newlineAntimicrobial resistance AMR is a complex global public health challenge the development of Drug resistance is a natural phenomenon in microorganisms Klebsiella pneumoniae is a Gram negative and rod shaped bacteria that belongs to the family Enterobacteriaceae Most of the positive isolates obtained from pus samples 100 35 point 34 percent There was a male predominance149 52 point 66 percent among the isolates obtained from the patients and the female was 70 24 point 73 percent The maximum number of positive isolates were from the patients in the age group of 41 to 60 years 19 point 08 percent The main mechanism of antibiotic resistance is production of beta lactamase that hydrolyse the beta lactam antibiotics The Carbapenems are the beta lactams that binds to the penicillin proteins and inhibits the synthesis of the bacterial cell wall Out of n equal 283 samples 219 isolates are Multi drug resistance of Klebsiella pneumoniae 219 strains are both ESBLs and Carbapenems ESBL strains were resistant to Cefotaxime n equal 219 and Carbapenems resistant to Imipenem n equal 138 Phenotypic identification method for ESBLs Combined Double disc method Phenotypic identification method for Carbapenems CarbaNP test mCIM and eCIM test respectively Multiplex Real time PCR was applied for the detection of ESBL genes SHV CTX M and TEM In present study out of 283 samples ESBL genes highest Positive was observed for CTX 175 61 point 84 percent followed by SHV 145 51 point 24 percent and TEM 121 42 point 76 percent Carbapenemase genes blaNDM blaKPC bla OXA48like blaIMP blaVIM Bla NDM was most prevalent gene followed by blaOXA48 Bla KPC blaVIM blaIMP genes were not found in any of the isolates newline newline