Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/579693
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dc.coverage.spatialMolecular pathogenesis of enterotoxaemia in neonatal goat kids
dc.date.accessioned2024-07-30T10:20:38Z-
dc.date.available2024-07-30T10:20:38Z-
dc.identifier.urihttp://hdl.handle.net/10603/579693-
dc.description.abstractIn this study, a total population of 1156 neonatal goat kids was examined for clinical diarrhoea newlineand 134 necropsied were examined for enteritis, pneumo-enteritis, tapeworms/round worm infestations newlineand septicemia, suspectedly died due to enterotoxemia from January, 2015 to December, 2016. A total newlineof 238 diarrhoeic fecal and 84 intestinal loop samples were collected from ICAR-CIRG, Makhdoom, newlineMathura (UP) and spontaneous outbreaks of Uttar Pradesh and Rajasthan States of India. Isolation, newlineidentification and molecular detection of toxinotypes of Clostridium perfringens were performed in newline238 diarrhoeic samples and 84 intestinal loop samples for confirmatory diagnosis of enterotoxemia. newlineDiarrhoeic samples and intestinal contents were used for isolation in Robertson cooked meat media newline(RCMM) and subsequently inoculated in 5% defibrinated sheep blood brucella agar with vitamin K1, newlinehemin and selective clostridial supplements (CLS-BBA) and egg yolk agar (EYA) under anaerobic newlineconditions. Toxinotyping were done by multiplex PCR using toxin genes viz., cpa, cpb, cpb2, etx and newlineiap. After confirmatory diagnosis, molecular characterization for different isolates was done by PCR newlinecloning and gene sequencing. The etx-full gene cloned in TA vector for various field isolates was newlinesequenced by using Bigdye® newline terminator v1.1 cycle sequencing kit (Applied biosystems). Phylogenetic newlineanalysis was conducted by minimum evolution tree analysis. Experimental study using characterized newlineisolate (CIRG-1816) was undertaken to study molecular and gene expression profiles. The inoculum newlinewas prepared in RCMM and culture supernatant (CS) containing epsilon toxin was trypsinized and newlinetitrated in mouse. Twenty male Jakhrana goat kids of 0-3 month s age weighing 9-12 kg were divided newlinein four equal groups (n=5). Group I was treated with CS, group II with washed cells (WAC), group III newlinewith whole culture (WC) and group IV with only RCM medium (control). By laparotomy, 20% starch newlinesolution was infused into abomasum and 300 ml of inoculum into duo
dc.format.extent151p
dc.languageEnglish
dc.relationU.P. Pandit Deen Dayal Upadhyaya pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU) Mathura Uttar Pradesh -281001
dc.rightsuniversity
dc.titleMolecular pathogenesis of enterotoxaemia in neonatal goat kids
dc.title.alternative
dc.creator.researcherSingh, Desh Deepak
dc.subject.keywordImmunology
dc.subject.keywordLife Sciences
dc.subject.keywordPathology cause of disease
dc.description.note
dc.contributor.guidePawaiya, Rajveer Singh
dc.publisher.placeMathura
dc.publisher.universityU.P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidhyalaya evam Go Anusandha Sansthan
dc.publisher.institutionVeterinary Pathology
dc.date.registered2014
dc.date.completed2017
dc.date.awarded2017
dc.format.dimensionsA4
dc.format.accompanyingmaterialCD
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:Veterinary Pathology

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01_title.pdfAttached File306.03 kBAdobe PDFView/Open
02_prelim.pdf4.98 MBAdobe PDFView/Open
03_content.pdf15.77 kBAdobe PDFView/Open
04_abstract.pdf19.1 kBAdobe PDFView/Open
05_chapter 1.pdf40.93 kBAdobe PDFView/Open
06_chapter 2.pdf240.06 kBAdobe PDFView/Open
07_chapter 3.pdf436.85 kBAdobe PDFView/Open
08_chapter 4.pdf5.8 MBAdobe PDFView/Open
09_chapter 5.pdf148.97 kBAdobe PDFView/Open
10_annexures.pdf1.09 MBAdobe PDFView/Open
11_chapter 6.pdf46.08 kBAdobe PDFView/Open
80_recommendation.pdf12.82 MBAdobe PDFView/Open


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