Evaluation of excretory secretory antigen as a candidate for serodiagnosis of caprine amphistomosis

Abstract

A study was conducted for the molecular characterization and development of serodiagnostics newlinefor caprine amphistomosis. A total of 92 rumens of goats naturally infected with amphistome species newlinewere collected from Army Slaughter House, Mathura (U.P). Beside rumen, blood samples were also newlinecollected from 11 newly born kids of age upto 1 week having stall feeding history of their dam and 240 newlinegoats by random sampling from nearby villages of Mathura district. Prevalence of amphistomosis by newlinerumen examination was divided in three major groups viz. heavy, moderate and light infection. Month newlinewise prevalence observed that the total maximum prevalence was found in month of July (21.87%) newlinefollowed by September (19.15%) and May (17.18%). The minimum prevalence of amphistomosis was newlineobserved in month of December (8.73%) followed by November (9.44%) and January (11.60%). Years newlineof study were also divided into three seasons viz. winter (Nov Feb), summer (March June) and newlinemonsoon (July October). The highest overall prevalence of amhistomosis on the basis of rumen newlineexamination was reported in summer season (17.83%) followed by monsoon season (16.64%) and newlinewinter season (10.53%). The prevalence of amphistomosis on the basis of faecal examination (n= 240) newlinerevealed 17.08% during August to October 2016. Female goats (19.20%) were found more susceptible newlineto amphistomosis in comparison to male goats (11.11%). On the basis of morphology and molecular newlinetechnique using amplification of ITS2 gene, three species of amphistomes viz. Paramphistomum newlineepiclitum (95.60%), Gastrothylax crumenifer (3.20%) and Fischoederius spp. (1.08%) were identified newlinefrom rumen. The amplicons of about 400bp was obtained by amplification of ITS2 gene with used newlineprimer. A total of 04 representative amplicons were sequenced by outsourcing. The sequences were newlinesubmitted in NCBI and the accession number (Paramphistomum epiclitum isolate mtjamp1.1: newlineKX657873; Paramphistomum epiclitum isolate mtjamp1.3: KX657875; Fischoederius sp.: KX639720 newlineand Gastrothylax crumeni