Isolation of bioactive phytoconstituents and formulation development of commelina benghalensis

Abstract

quotAim: The purpose of the present study was to investigate ethnomedicinal claims, isolate bioactive phytoconstituents, and develop an effective pharmaceutical formulation from C. benghalensis, thereby making Best from Waste . newline newlineMaterials and Methods: Different extracts were prepared from the powder of the whole plant by using successive extraction method (Pet. Ether, Chloroform, Methanol and Water) followed by pharmacological screening (Laxative, Antiulcer activities) by using different animal models. This was followed by GC-MS, VLC, TLC, Column Chromatography, HPTLC Fingerprinting, Preparative TLC, FTIR, and 13C NMR of isolated fractions. A suitable formulation (effervescent granules) was prepared, evaluated, and subjected to in vivo animal activity. newline newlineResult and Discussion: Methanolic extract of C. benghalensis was found to contain alkaloids, flavonoids, saponins, steroids, tannins, phenolics, and carbohydrates. newlineIn vivo, laxative and antiulcer animal activity was performed by using the Loperamide-induced constipation model and ethanol-induced ulcer model, respectively, in male Wistar rats. Based on findings, hydroalcoholic extract showed significant laxative as well as antiulcer activity. Afterwards from the bioactive extract effervescent granules were prepared, which passed official IP and BP testing and showed significant in vivo animal activity. For identification of bioactive compound GC-MS was performed. GC-MS of the CB/MEOH extract showed 19 bioactive phytoconstituents. For further isolation, VLC of CB/MEOH was performed which made 3 fractions from which CB/F3 fraction was found more potent by being subjected to TLC by using a solvent system (optimization). Potent CB/F3 fraction was subjected to Column chromatography using different proportions of Chloroform and Methanol and made 120 fraction. From that, Fraction F41 was found with an isolated compound. For identification, Fraction F41 was subjected to HPTLC fingerprinting which showed different Rf values at 366 nm. Identification of compound in F41 done by GC-