Validation of Ethanomedicinal Claims of Medicinal Plant and Bioactive Phytochemical For Antiangiogenic and Anticancer Activity

Abstract

Aim: The present study aims to Validation of Ethanomedicinal Claims of Medicinal Plant newlineand Bioactive Phytochemical for Antiangiogenic and Anticancer Activity . newlineBackground: Currently, there is a growing interest towards use of natural antioxidants and newlineanti-inflammatory compounds obtained from herbal sources in the treatment of metastatic newlinecancer. Different epidemiological survey, in vitro and in vivo studies related with plants newlinehave confirmed that phytoconstituents having antioxidant, anti-inflammatory and newlineantiangiogenic activity have ability to suppress tumor development in humans. Numerous newlineplant-derived bioactive compounds have been documented for their potential in both newlinepreventing and treating cancer like kaempferol, luteolin, quercetin, genistein, curcumin etc. newlineThese biologically active phytoconstituents produces action by different mechanisms and newlinemight be responsible to produce action on more than one target like inhibitory effect on newlineoxidative damages, inflammation process, tumor cell angiogenesis and cancer cell line newlinegrowth. For the discovery of new anticancer agents herbal medicines are supposed to be newlinethe best option. To our understanding, there exists no other research documenting the antiangiogenic newlineand anticancer properties of Citrus karna Raf. fruit peel. newlineMethods: The Citrus karna Raf. fruit peel powder was extracted by Soxhlet extractor using newlinepet ether, methanol, ethanol, acetone and separately by water. The pet ether (CKPE), newlinemethanol (CKME), ethanol (CKEE), acetone (CKAE), and water (CKWE) extracts were newlineobtained. All the extracts were investigated for the presence of different phytochemicals newlineusing preliminary phytochemicals, TLC, total phenolic and flavonoid content. The newlineassessment of total phenolic content (TPC) and total flavonoid content (TFC) was newlineconducted employing a spectroscopic method. newlinexxii newlineThe antioxidant potential of all the extracts of peels of Citrus karna Raf. was newlineevaluated using five antioxidant assay namely DPPH (1, 1- diphenyl-2-picrylhydrazyl) newlineradical scavenging activity, hydrogen pero