Study On Neuroprotective Role of Curcuma Longa L Against Benzo A Pyrene Induced Neurodegeneration In Zebrafish Danio Rerio Hamilton 1822 By Improving Brain Antioxidant Status

Abstract

ABSTRACT newlineOxidative stress comprises an important aspect of toxicology and is also of ecological newlinesignificance, particularly in the aquatic environment. The aquatic ecosystem provides a sink for newlinethe persistent organic environmental pollutants that have potential to induce oxidative stress by newlinepromoting neurobehavioral, biochemical, and neuromorphological alterations in aquatic animals. newlineOrganic pollutants are found at higher concentrations at higher trophic levels in food chain and newlineare dangerous to human health resulting in anxiety-like behavior. Oxidative stress caused due to newlinebenzo[a]pyrene, a prototype of high-molecular weight polycyclic aromatic hydrocarbons, is newlineassociated with several neurodegenerative disorders. The main purpose of this study is to newlineelucidate neurotoxic potential of benzo[a]pyrene (B[a]P) and neuroprotective role of Kandhamal newlinehaladi (Curcuma longa L.) against benzo[a]pyrene-induced neurotoxicity by improving zebrafish newlinebrain antioxidant status. Zebrafish (Danio rerio Hamilton, 1822) are extremely sensitive to newlinevariety of environmental stresses, have a number of traits similar to rodent model and have 70% newlinefunctional similarity with human disease genes. This supports the validity of zebrafish as a newlinemodel for studying acute anxiety-like behaviour. Curcuma longa L. used in Ayurveda to treat a newlinenumber of neurodegenerative diseases. Curcumin, a phytochemical present in Curcuma longa L. newlinehas antioxidant effect and it protects against neurological and anxiety-related disorders. This newlinestudy aimed at evaluating the B[a]P-induced neurotoxicity following waterborne exposure in newlineadult zebrafish (Danio rerio Hamilton, 1822) model and neuroprotective efficacy of Kandhamal newlinehaladi (Curcuma longa L.) co-supplementation against B[a]P-induced neurotoxicity. Two widely newlineused tests for behavioral study of zebrafish are light and dark box test (LDBT) and novel tank newlinexxix newlinediving test (NTDT). LDBT is based on the tendency of adult zebrafish to seek dark environment newlinewhen exposed to new environmental conditions. NTDT is based on th