Isolation and in Vitro Evaluation of Bacteriophages against Mdr Escherichia Coli Isolation from all Clinical Specimens

Abstract

Introduction: Multi-drug-resistance (MDR) is a severe public health concern worldwide, and its containment is more challenging in developing countries due to poor antimicrobial resistance (AMR) surveillance and irrational use of antibiotics. The practice of phage therapy, which uses bacterial viruses (phages) to treat bacterial infections, has been around for almost a century. The universal decline in the effectiveness of antibiotics has generated renewed interest in revisiting this practice. Aims and Objectives: The aim of this study is ISOLATION AND IN VITRO EVALUATION OF BACTERIOPHAGES AGAINST MDR ESCHERICHIA COLI: ISOLATION FROM ALL CLINICAL SPECIMENS . Material and Methods: The study was conducted at the Department of Microbiology, RKDF Medical College Hospital and Research Centre, SRK University, located in Bhopal, Madhya Pradesh. This study was carried out after the approval of Institutional Ethical Committee. Duration of study was over a period of two years after the approval. The study was performed on 785 patients within the age group 10 to 70. The study subjects were reported from both the Out-Patients Department and In-Patients. A valid written informed consent was taken from each patient. This study divided in two part: isolation of clinical isolates (MDR E. coli) and isolation of Environmental isolates (Bacteriophages against MDR E. coli). Out of 785 isolates we were identified 140 Escherichia coli. Among the 140 Escherichia coli, 122 were MDR Escherichia coli and rest were normal E. coli. After that, Bacteriophages (environmental isolates) were prepared against 122 MDR Escherichia coliand see the in vitro lytic efficacy of bacteriophages against MDR Escherichia coli. Result: Total 785 cases were included in this study. Among all 140 cases were infected with E. coli. Out of 140 E. coli,122 (87.2%) MDR E. coli were seen. Majority of samples vii were urine samples followed by pus, blood, swab and fluid sample. It is found that there were a number of antibiotics which were resistant to Ampicillin, Tetracyclin