Formulation and Evaluation of Novel Probiotic Based Serum for the Treatment of Acne

Abstract

Acne refers to a multifactorial dermatological disorder affecting people of newlineall age groups especially adolescents. Different strategies used for acne treatment newlineincludes the topical and oral use of antimicrobials, hormonal therapy and physical newlinetreatments. The world pharmaceutical market is flooded with a range of pharmaceutical newlineantiacne products. But the conventional anti acne products are related with a range of newlineadverse effects like redness, itching, resistance etc. This leads to a quest for a better newlinealternative. The present research work aims at using bacteriocins as the active newlinecompound. Bacteriocins are the proteinaceous compounds obtained preferably from the newlineprobiotics belonging the Lactobacillus family. Bacteriocins are well known for their newlineantimicrobial activity and safety. However, the commercial/industrial production of newlinebacteriocin is facing several limitations. Short stability period and low yield are the newlinemajor ones. Fusion of bacteriocins with nanoparticles proves to be a novel strategy to newlineovercome such hurdles. Additionally, serum is a better topical formulation rather than newlinecreams and gels as it offers better skin penetration and nutritional properties. So, the aim newlineof this research work was to incorporate bacteriocin fused silver nanoparticles, newlinemicrospheres containing coencapsulated probiotics with prebiotics and a natural newlinesurfactant (Soaproot extract) having good antiacne properties in the formulation. newlineSerum is the formulation ofinterest enriched with nutrients like essential oils, vitamins newlineetc. Both invitro and in vivo characterization studies were done using techniques such newlineas UV- Visible spectroscopy, disc- diffusion method (antimicrobial activity), SEM, newlineMicrobial identification (phenotypic methods), Pour plate method and skin irritation newlinestudies on rabbits newline