Production purification and characterization of biosurfactant from environmental bacterial isolate and its application in treatment of MRSA induced murine burn wound infection

Abstract

The study marks a pioneering exploration into the therapeutic potential of biosurfactants within a burn wound infection model. In this study biosurfactant production was optimized from environmental bacterial isolate from oil spillage sites. Which are the common sites for the biosurfactant producing microorganisms. Here in the present study we explored its antibacterial potential which was confirmed by minimum inhibitory concentration and Field emission scanning electron microscope (FESEM). Specifically, their efficacy as an antibacterial agent against MRSA 43300 ATCC, both independently and in conjunction with the antibiotic vancomycin was evaluated in treatment of murine burn wound infection. By introducing biosurfactants as an alternative therapeutic avenue, this research addresses the pressing challenge of antibiotic resistance prevalent in microbial communities. Furthermore, the combination therapy approach not only harnesses the antibacterial properties of biosurfactants but also mitigates antibiotic pressure. Given the well-documented role of biosurfactants in facilitating wound healing through skin regeneration, their incorporation into this study was a natural fit. The observed clearance of infection underscores their antibacterial efficacy, the rationale behind their integration into the experimental framework. Ultimately, the findings affirm the superior effectiveness of combined therapy (biosurfactant and antibiotic) over individual modalities, underscoring the potential of biosurfactants to revolutionize treatment paradigms for burn wound infections. newline