Phytochemical Screening isolation and Characterization of Bioactive Component from medicinal Plant of Satpura Region with Special Reference to Anti Urolithiasis Activity

Abstract

The folklore medicinal plants Equisetum ramosissimum and Selaginella bryopteris were selected for the study. In the present work phytochemical and Antiurolithiasis studies of Equisetum ramosissimum and Selaginella bryopteris with characterization of bioactive component was done. Petroleum ether, Ethyl acetate and methanol extracts of Equisetum ramosissimum whole plant and Selaginella bryopteris whole plant were taken to carryout qualitative phytochemical studies, which revealed the presence of various phytochemicals. The total phenolic, flavonoid contents were estimated in ethyl Acetate and methanol extract of both the plants. In this study Quantitative phytochemical screening method of crude extracts was performed through Total Phenolic Content [TPC] and Total Flavonoid Content [TFC] assays which confirmed the presence of Phenolics and Flavonoids in plant material. The bioactive components ER-1 from methanol extract of Equisetum ramosissimum whole plant and SB-2 from methanol extract of Selaginella bryopteris whole plant were isolated by using column chromatography and thin layer chromatography. The structures of these bioactive components were established by the use of IR, UV-VIS, NMR and Mass spectroscopy. ER-1 was characterized as and#946;-sitosterol and SB-2 as Amentoflavone. The Mass spectrum confirm the molecule isolated is Amentoflavone since M-1 peak appears at m/e ratio of 538 M+ and molecular weight of Amentoflavone is 538.45 g/mol. In the present study we investigated Antiurolithiasis activity of Equisetum ramosissimum and Selaginella bryopteris against ethylene glycol and ammonium chloride and ethylene glycol induced urolithiasis in rats. Today, Herbal therapies of preventing kidney stones have gained importance in the last decade. Ethylene glycol and ammonium chloride for 21 days was used for induction of urolithiasis and the curative effects of Equisetum ramosissimum and Selaginella bryopteris were checked for the level of oxalate, calcium, creatinine, uric acid, and urea of both urine and serum. Treatmen