In silico identification of regulatory genes during meiotic process in Saccharomyces cerevisiae

Abstract

This thesis incorporates in-silico methods to elucidate important molecular events leading to switch from mitosis to meiotic growth in S. cerevisiae. It unravels probable regulatory cascades by delineating important Master Regulators (MRs), Transcription Factors (TFs) and their Target genes (TGs) for three pivotal stages during meiotic growth in S. cerevisiae i.e. pre-meiotic, meiosis initiation and meiosis commitment stages. Lastly, it reports that the transcriptional cascade for meiosis commitment is more evolutionary conserved compared to transcriptional cascade for meiosis initiation phase in lower eukaryotes like S. cerevisiae and higher eukaryotes like M. musculus. This thesis sheds light into the regulatory mechanisms of three important phases during meiotic growth of S. cerevisiae which will be helpful for gaining intricate knowledge of the meiotic process in eukaryotes. newline