Tissue interaction characterization of nanonutrients effect on metabolism and immune modulation in developing chicken embryo

Abstract

The present study has been designed on analysing nanoparticle tissue interaction during embryonic development which is of contemporary need on evaluation of nanobiomolecules in in vitro and in vivo models. Chromium and Zinc were the minerals chosen for the analysis of this study. Chromium in its trivalent form is considered as the most stable one compared to the other chromium oxides. The most important method of achieving this is by the green synthesis of the macro chromium with plant biogenic phyto-molecules. Similarly, zinc is an important supplement in the poultry diet which plays an essential part in the growth, immune and reproductive systems. The current study was carried out by green synthesis of chromium and zinc nanoparticles and characterizing by particle size analysis, UV-vis spectroscopy, scanning electron microscopy, Zeta potential, X-ray diffraction and Fourier Transform Infrared spectroscopy. These nanoparticles were further analysed by finding out the effect in vitro in different cell cultures and finding out a suitable dosage for its extrapolation in in vivo studies. The in vivo study was carried out in chorioallantoic membrane of the developing chicken embryo and further analysed by tissue interaction studies of chromium and zinc nanoparticles on various tissues of the developing chicken embryo in various stages of the development until hatch for metabolism and immunomodulation. Bioavailability of these nanoparticles was also assessed on various stages of development. These findings carried out with objectives would facilitate the contemporary need for researchers and institutions engaged in screening biomolecules and nanomolecules for biomedical and food safety research in developing embryo model. This could also be a gap filling on the need for an ideal in vivo model as a test system for evaluating any biomolecule. newlineKeywords: Nanoparticles-in vitro-in vivo-tissue interaction-cell culture - CAM - bioavailability quantitative PCR newline newline