In vitro and in vivo analysis of curcumin loaded amine functionalized SBA 15 incorporated polymeric nanofibrous scaffold for skin wound healing application

Abstract

Nano-biomaterials are applied as polymeric scaffolds in skin wound healing newlineapplication and various field of tissue engineering. The electrospinning technique is the newlinesimplest method for the fabrication of nanofibers. Electrospun PVA and PCL based newlinenanofibrous scaffolds can provide properties such as good surface morphology, inter newlineconnectivity, dense fiber orientation, mechanical strength, moisture retention, newlinebiocompatibility, cell adhesion property and mimicking the natural Extra Cellular newlineMatrix (ECM) for the regeneration of injured tissue in wound healing application. On newlinethe other hand, mesoporous silica has unique properties such as a large surface area to newlinepore volume, tunable pore size, chemical surface modification, thermal stability, newlinebiocompatibility, hydrophilic surface, and drug-carrying ability. Hence, a combination newlineof both nanofiber and mesoporous silica material could facilitate any desired antibiotic newlineand antibacterial drug to attain an improved biological response. Additionally, newlinetraditional therapeutic drugs and their biological properties encourage wound healing newlineactivity. Hence, in the present study, curcumin was loaded in the mesoporous silica newlineincorporated polymeric solution and nanofibrous scaffolds were fabricated and used as newlinea wound healing biomaterial. The developed nanofibrous scaffolds were characterized newlinethrough XRD, FTIR, SEM and EDAX and the properties such as biocompatibility, cell newlineviability, cell adhesion property, antibacterial property, drug release profile and wound newlinehealing efficacy were studied through in-vitro and in-vivo rat model. Histophathalogical newlinestudy was used to determine the the recovery of wound using HandE staining and newlineMasson s trichrome staining. newlineInitially, SBA-15 is synthesized using pluronic123, a structure-directing newlineagent, and tetraethylorthosilicate as a silica source. Then the synthesized SBA-15 is newlinecharacterized by XRD, FTIR, SEM, TEM and BET analysis. newline