1. Shodhganga@INFLIBNET
  2. Panjab University
  3. University Institute of Engineering and Technology
Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/545782
Title: Molecular and Phenotypic Studies to Characterize Dhhal3 from Halotolerant Yeast Debaryomyces hansenii
Researcher: Sharma, Aditi
Guide(s): Puri, Sanjeev and Minhas, Anu Priya and Jaspreet Kaur
Keywords: Debaryomyces hansenii
Dhhal3
Flavoprotein
University: Panjab University
Completed Date: 2023
Abstract: Dhhal3 gene from halotolerant yeast, Debaryomyces hansenii was identified as 1670bp gene translating to 559 amino acids long flavoprotein. 6XHis tagged DhHal3 was bacterially expressed as~73kDa, moderately thermostable protein exhibiting decarboxylase activity. Dhhal3 over expression in D. hansenii exhibited salt stress tolerance phenotype upto 0.4M LiCl and 1.5M NaCl salt concentration while dhhal3 null mutant exhibited a sensitive phenotype. Dhhal3 overexpressing Arabidopsis plant survived 150mM NaCl salt concentration. Overall, Dhhal3 from D. hansenii exhibited the potential to develop salt tolerant crops in the future. newline
Pagination: vi, 105p.
URI: http://hdl.handle.net/10603/545782
Appears in Departments:University Institute of Engineering and Technology

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01_title page.pdfAttached File40.6 kBAdobe PDFView/Open
02_prelime pages.pdf1.51 MBAdobe PDFView/Open
03_chapter 1.pdf69.27 kBAdobe PDFView/Open
04_chapter 2.pdf378.46 kBAdobe PDFView/Open
05_chapter 3.pdf484.34 kBAdobe PDFView/Open
06_chapter 4.pdf2.75 MBAdobe PDFView/Open
07_chapter 5.pdf91.39 kBAdobe PDFView/Open
08_chapter 6.pdf80.71 kBAdobe PDFView/Open
09_annexure.pdf151.36 kBAdobe PDFView/Open
80_recommendation.pdf116.9 kBAdobe PDFView/Open
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