Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/545782
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dc.coverage.spatialBiotechnology
dc.date.accessioned2024-02-19T05:23:20Z-
dc.date.available2024-02-19T05:23:20Z-
dc.identifier.urihttp://hdl.handle.net/10603/545782-
dc.description.abstractDhhal3 gene from halotolerant yeast, Debaryomyces hansenii was identified as 1670bp gene translating to 559 amino acids long flavoprotein. 6XHis tagged DhHal3 was bacterially expressed as~73kDa, moderately thermostable protein exhibiting decarboxylase activity. Dhhal3 over expression in D. hansenii exhibited salt stress tolerance phenotype upto 0.4M LiCl and 1.5M NaCl salt concentration while dhhal3 null mutant exhibited a sensitive phenotype. Dhhal3 overexpressing Arabidopsis plant survived 150mM NaCl salt concentration. Overall, Dhhal3 from D. hansenii exhibited the potential to develop salt tolerant crops in the future. newline
dc.format.extentvi, 105p.
dc.languageEnglish
dc.relation-
dc.rightsuniversity
dc.titleMolecular and Phenotypic Studies to Characterize Dhhal3 from Halotolerant Yeast Debaryomyces hansenii
dc.title.alternative
dc.creator.researcherSharma, Aditi
dc.subject.keywordDebaryomyces hansenii
dc.subject.keywordDhhal3
dc.subject.keywordFlavoprotein
dc.description.noteBibliography 85-105p.
dc.contributor.guidePuri, Sanjeev and Minhas, Anu Priya and Jaspreet Kaur
dc.publisher.placeChandigarh
dc.publisher.universityPanjab University
dc.publisher.institutionUniversity Institute of Engineering and Technology
dc.date.registered2015
dc.date.completed2023
dc.date.awarded2024
dc.format.dimensions-
dc.format.accompanyingmaterialCD
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:University Institute of Engineering and Technology

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01_title page.pdfAttached File40.6 kBAdobe PDFView/Open
02_prelime pages.pdf1.51 MBAdobe PDFView/Open
03_chapter 1.pdf69.27 kBAdobe PDFView/Open
04_chapter 2.pdf378.46 kBAdobe PDFView/Open
05_chapter 3.pdf484.34 kBAdobe PDFView/Open
06_chapter 4.pdf2.75 MBAdobe PDFView/Open
07_chapter 5.pdf91.39 kBAdobe PDFView/Open
08_chapter 6.pdf80.71 kBAdobe PDFView/Open
09_annexure.pdf151.36 kBAdobe PDFView/Open
80_recommendation.pdf116.9 kBAdobe PDFView/Open


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