Design and Synthesis of Novel Chemodosimetric Probes and Their Photophysical and Bioimaging Studies with Analytes

Abstract

Revolutions in science and technology have greatly improved our lives.Over the last decades, fluorescentbioimaging technology is one of the most popular and fastest growing areas inbiomedicine and bioanalysis. Recent research suggests that bioactive species, including reactive oxygen species (HOCl), reactive sulfur species (Cys, H2S) production promotes both viral replication and monocyte activation including Coronavirus infection.Therefore, development of highly selective, low cost and infield application of fluorescent chemodosimeter have to be required to detect bioactive species in different media. In this study we take organic molecule (host) possessing convergent binding site and guests are anion, cation and molecule containing divergent binding site. newlineIn this dissertation entitled Design and Synthesis of Novel Chemodosimetric Probes and Their Photophysical and Bio-imaging Studies with Analytes covers the design, synthesis, spectral studies in different media, DFT-TDDFT studies, fluorescence lifetime studies,cell imagingandsurface morphologystudies of Chemodosimetricprobe.These novel probecan sensingbioactive specieslike hydrogen sulfide(H2S), hypochlorite (HOCl-), cysteine,intracellular viscosity andlethal hydrazine(N2H4)also. newlineIn Chapter 1,a short introductionabout the basic principle of molecular recognition andsupramolecular chemistryincluding its definition and interdisciplinary nature. This chapter contains different modes of non-covalentinteraction that are responsible during the course of host-guest complex formation. Different mechanism of different sensing phenomena correlated with published workalso has been discussed in this part.Some innovative applications of the concepts of molecular recognition and supramolecular chemistry areincorporated in this chapter. newlineIn Chapter 2, a novel ratiometric fluorescent probe 3-IA wasdesignforthedetection of hydrogen sulfide (H2S)in biological systems. In 3-indolylacrylate derivative, acrylate moiety acts as the Michael acceptor towards H2S and the resultant