In vivo evaluation of efficacy and saftey of ethanolic seed extract of prosopis juliflora in fructose induced hyperglycemia in male albino rats

Abstract

Aim: newlineEvaluation of the effectiveness as well as safety of an ethanolic extract of seeds derived from the Prosopis juliflora plant in male Wistar albino rats with fructose-induced diabetes. newlineObjective: newlinea. To evaluate the effectiveness of an ethanol-based extract of seeds derived from the Prosopis juliflora plant using the next criteria for measuring results: newlinei. Fasting blood sugar (FBS), fasting insulin, including the HOMA- (Homeostatic model evaluation for insulin resistance) newlineii. Measurement of lipid profile iii. Assessment of physical size newlineb. To evaluate the safety of the ethanolic seed extract of Prosopis juliflora using the specified outcome measures: newlinei. Full blood count (FBC) ii. Renal function panel (RFP) iii. Hepatic function panel (HFP) newlineiv. Indicators of toxicity newlinev. Death rate newlineMethodology: newlineThirty male Wistar albino rats, weighing between 190-200g, were chosen and evenly distributed into six groups. for the disease. Groups III to V were the experimental groups receiving different treatments. The animals in group I were provided with conventional drinking water, whereas groups II to V were provided with 20% fructose water in addition to a standard pellet diet for a duration of 8 weeks. If the animals consumed all the fructose water, they were given regular drinking water for the remainder of the day. After a duration of 8 weeks, all the mice exhibited characteristics indicative of and HOMA-IR data corroborate our observation. Furthermore, the addition of fructose supplementation not only resulted but also caused some characteristics of metabolic syndrome such as increased body weight newlineand changes in lipid levels. During weeks 9 to 16, the animals underwent the following interventions: group I received regular drinking water, group II received 2ml III, group IV received P.juliflora received dosage of 600mg The interventions were diluted in 2ml of distilled water for administration. Measurements were taken for body weight. newlineResults: newlineAnimals belonging to groups II to V exhibited characteristics dyslipidemia. newlineAnimals of 200mg of metformin, and groups IV and V, treated 400mg and 600mg of P.juliflora , respectively, exhibited a notable reduction in levels of FBS, HOMA-IR, a significant in when compared to the disease control group. newlineAlthough both doses notable, the 600mg/kg dose had the highest efficacy, comparable to that of the conventional medication metformin. The metformin and extract treated groups had similar decreases in, along with an levels. newlinenalysis the group metformin and dosage 600mg/kg showed the presence normal islet cells with pale and ovoid beta cells. The disease control group exhibited the existence of islet cells accompanied by an inflammatory infiltration. The group dosage o400mg exhibited normal islet cells with little degenerative alterations. These data suggest that the islet cells that were injured may have undergone recovery following treatment with the plant extract. newlineConclusion: newlineEthanolic seed extract of P.juliflora in the dose 600mg/kg showed significant antidiabetic activity which was equivalent to the effect produced by metformin. While, metformin and P.juliflora seed extract in the doses 400mg/kg and 600mg/kg showed comparable antidyslipidemic activity. newline