Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/540451
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dc.coverage.spatialDepartment of Biology
dc.date.accessioned2024-01-18T12:14:39Z-
dc.date.available2024-01-18T12:14:39Z-
dc.identifier.urihttp://hdl.handle.net/10603/540451-
dc.description.abstractPost-translational modification of a substrate protein by SUMO (Small Ubiquitin-related modifier) can modify its activity, localization, interaction or function. A large number of SUMO targets in cells have been identified by proteomic studies, but the biological roles for SUMO conjugation for most targets remain elusive. Multi-aminoacyl tRNA Synthetase complex (MARS) is a large cytoplasmic 1.2 MDa signalling hub that acts as a sensor and regulator of the immune response. MARS consists of eight Amino-acyl tRNA Synthetases (AARS) and three non-synthetase adaptors (AIMP1-3). Using quantitative proteomics, we have determined that the members of the MARS complex showed enhanced SUMO conjugation in response to an immune challenge (Handu et. al., 2015). Subsequently, I could demonstrate that eight of its eleven members were SUMO conjugated using in-vitro SUMOylation assays. Immunoprecipitation of the MARS complex, followed by mass spectrometry, suggests that the complex was stabilized in response to both gram-positive and gram-negative infection in adult flies, underscoring a role for MARS in the Drosophila immune response. Glutamyl-Prolyl tRNA Synthetase (EPRS), a member of MARS sub-complex I is SUMO conjugated at its WHEP domain, which is involved in non-canonical roles. In mammals, EPRS dissociates from the MARS complex in response to infection, to form a secondary GAIT complex, that regulates translation. In order to study roles for SUMO conjugation of EPRS, I have used CRISPR Cas9 genome editing technology to generate a SUMO conjugation resistant (SCR) variant (EPRSSCR; EPRSK957R, K1063R, K1083R, K1106R, K1198R). The transgenic lines generated were unstable, precluding the exploration of immune regulation in EPRSSCR flies. Arginyl tRNA Synthetase (RRS), a member of sub-complex II of MARS is also SUMO conjugated. A SCR variant (RRSSCR; RRSK147R,K383R) was uncovered by a combination of in-bacto SUMOylation assay with Lys mutagenesis. Transgenic Drosophila lines of RRSWT and RRSSCR were made by expressing
dc.format.extentNA
dc.languageEnglish
dc.relationNA
dc.rightsself
dc.titleRegulation of the drosophila innate immune response by sumo conjugation of amino acyl trna synthetases
dc.title.alternativeNa
dc.creator.researcherNAYAK, PRAJNA
dc.subject.keywordBiology
dc.subject.keywordBiology and Biochemistry
dc.subject.keywordLife Sciences
dc.description.noteNA
dc.contributor.guideRATNAPARKHI, GIRISH S.
dc.publisher.placePune
dc.publisher.universityIndian Institute of Science Education and Research (IISER) Pune
dc.publisher.institutionDepartment of Biology
dc.date.registered2014
dc.date.completed2023
dc.date.awarded2023
dc.format.dimensionsNA
dc.format.accompanyingmaterialNone
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:Department of Biology

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