Single Nucleotide polymorphisms in miRNAs Associated with Colorectal Adenocarcinoma Coverage Colorectal cancer microRNAs

Abstract

Colorectal cancer is one of the most widespread cancers worldwide accounting for high mortality MicroRNA polymorphisms have been associated with several cancers and the effect of these miRNA SNPs is known to vary with ethnicities The current case control study was set to analyze the role of miRSNPs that interfere with the miRNAmRNA regulatory network involved in inflammatory tumorigenesis and tumor angiogenesis in colorectal cancer patients Here a total of six miRSNPs rs10889677 rs16870224 rs696 rs2684788 rs12140 and rs3025039 were selected for the study The SNPs were genotyped in 200 colorectal cancer cases and 200 cancer free controls using TaqMan Allelic discrimination assay Association analysis showed that the Let 7f binding site SNP rs10889677 in IL23R gene and the miR 449a binding site SNP rs696 in NFKBIA gene was found to influence the risk of CRC where the latter displayed an increased risk Sub group analysis revealed that rs10889677 SNP has strong association with Well Differentiated and Poorly differentiated subgroups by increasing the risk of CRC and rs2684788 was associated with an increased risk of Well Differentiated Colorectal cancer MDR analysis showed that PTGER4 SNP IL23R SNP and IGF1R SNP were strongly interacting in the presence of sex parameter thereby increasing the risk of Colorectal cancer Immunohistochemical staining was done to analyze NFkB protein expression and was found to be increased in Moderately Differentiated subgroup To conclude the study revealed out the association of miRNA binding site SNPs with the risk of Colorectal cancer in the Indian Colorectal cancer patients newline