Oxidative Stress and Enzymatic Antioxidant Defense Systems With Special Reference to Vitamin E Supplementation in Exercising Females

Abstract

newline Background : Strenuous physical exercise is known to increase the production of reactive newlineoxygen species (ROS). Oxidative stress ultimately increases the likelihood of cellular oxidation, newlineassociated with depletion of antioxidant defense. The production of free radicals can be newlinecounteracted by catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and newlineglutathione (GSH), called antioxidant scavenger enzymes. Due to oxidative stress serum lipid newlineperoxidation level is also significantly increased after exercise, thus, enhancing the susceptibility newlineof erythrocyte to hemolysis, indicated by the activities of lactate dehydrogenase (LDH), acid newlinephosphatase (AP) in the serum. When the ROS production in the exercising muscle overwhelms newlinethe body s own antioxidant defense system then antioxidant supplementation is needed. Although newlineantioxidant supplementation are widely available and commonly used in the United States, there newlineis a lack of detailed information of their use, including types of supplementation used, doses, newlineduration of use and changes in use overtime. How the enzymatic defense and antioxidant vitamin newlinesupplementation are correlated and how these two oxidative stress management systems newlinecohesively balances the stress : strain ratio, particularly during recovery phase are yet not to be newlineworked out. Objectives: To elucidate the effect of physical exercise on antioxidant enzyme newlinesystems, blood glutathione and exercise induced lipid peroxidation status in female subjects with newlinereference to the oral administration of antioxidant and other vitamins with trace minerals newlinesupplementation at clinical dose and its effectiveness in controlling antioxidant enzymes, blood newlineGSH and lipid peroxidation in exercising females. Methodology: 250 female students of 18-21 newlineyears age were randomly chosen from Index Medical College, MP, for the study and divided into newline5 sub-groups (01 Placebo and 04 Experimental groups). Endurance capacity of each subject was newlinedetermined by exercising them in Magnetic Brake Bicycle Ergome