Studies on the invitro propagation of Exacum bicolor Roxb and evaluation of its bio active compounds for treatment of diabetes

Abstract

newline Diabetes mellitus is a progressive metabolic newlinedisorder arising due to the lifestyle changes and accounts for 9.3% of diseases newlineglobally. Though use of allopathic drugs alleviates the disease, it requires a prolonged newlinetreatment which leads to development of adverse effects. Hence, treatment using newlinemedicinal plants is gaining importance as it is cost effective and has fewer side newlineeffects. Exacum bicolor Roxb. is one such medicinal plant known to be used as a newlinetraditional medicine in treatment of diabetes. This is an endemic plant and now, is in newlinethe verge of being endangered. So, there is a compulsive need for protection of this newlineplant species. Hence, in-vitro propagation methods can be used to revive the newlinepopulation of E .bicolor. newlineIn this study, E. bicolor plantlets were developed by in-vitro propagation techniques newlineto evaluate its anti-diabetic potential. The E. bicolor plant was propagated under in- newlinevitro conditions from its leaves (explants) to obtain plantlets. These plantlets were newlineextracted in different solvents and analysed using various qualitative and quantitative newlinetests to identify its bioactive compounds (phytochemicals). Further, the role of these newlinephytochemicals in diabetic pathways was studied by using in-silico techniques. The newlineanti-diabetic potential of these extracts was validated by performing animal studies newlineusing diet induced obese mice. Meanwhile, the extracts were also evaluated for its newlineantioxidant and anti-microbial properties. newlineThus, we report an efficient protocol for in-vitro propagation of E. bicolor and the newlinepresence of two potent anti- diabetic phytochemicals that are promising compared to newlinethe commercial drugs being used in treatment of diabetes. Also, observing the positive newlineresponse of these extracts in animal studies has corroborated the anti-diabetic newlinepotential of E.bicolor Roxb.