Characterization and Optimization for large scale production of PGP attributes of Talaromyces trachyspermus as effective biofertilizer for soybean and wheat cropping system

Abstract

The present research was conducted with fungal culture Talaromyces trachyspermus 4014 (accession number MF509777) was procured from the Department of Microbiology, Barkatullah University Bhopal, M.P. India. Fungal culture was analyzed for immense production of indole acetic acid, siderophore, phosphate solubilization, zinc solubilization and ammonia production. It was also showed maximum antagonism against Fusarium oxysporum (ITCC 6859) among different phytopathogens. By the optimization of various PGP attributes for large scale production, IAA production was increased up to 0.78 fold and siderophore 0.33 fold by OVAT method. Moreover, phosphate solubilization was enhanced up to 1.41 fold by Plackett-Burman and Response Surface Methodology. Successful scale-up fermentation process for the phosphate solubilization and organic acid production was conducted in a 5L bioreactor and the solubilization of phosphate was maximized up to 1.55 fold from the initial value. Characterization of organic acids produced in culture filtrate of fungus was done by HR LC-MS ORBITRAP analysis. The Biofertilizer effect of Talaromyces trachyspermus fungus was seen on wheat and soybean crops. Maximum results were found in the treatments containing combinations of Talaromyces trachyspermus fungus and organic fertilizer (FYM). Biocontrol efficiency of fungus against Fusarium oxysporum (ITCC 6859) was found maximum in the combination of Talaromyces trachyspermus, Fusarium oxysporum, and 75% of recommended fungicide as compared to control treatment. From the present research method, it is indicated that plant growth-promoting fungus (PGPF) could be a potent microbial inoculant for sustainable agriculture system. newline