Rice transformation and expression profile of chloroplastic genes isolated from c4 species of poaceae

Abstract

Atmospheric concentrations of carbon dioxide have been steadily rising and are newlineprojected to rise further by the year 2100 (IPCC 2007). The increase in C02 concentration newlinegive rise to global wanning which has a reverse impact on growth rate and yield of plant. To newlineovercome this problem, biotechnological tools may play a significant role in creating such newlineplants with genetic modification so that they can become tolerant to this adverse condition. newlineRapid population growth, shrinkage of agricultural land, climate change and water scarcity newlineare the important factors for which yield potential of rice must be increased substantially to newlinefeed the world burgeoning population. Rice is an inherent part of human civilization and newlineconsumed by majority of Asian as their staple food. In addition to classical plant breeding newlinemethods, biotechnological tools including genetic engineering and genome editing are newlineimportant for rice improvement to meet the growing demand. Both genetic engineering and newlinegenome editing technology are dependent on in vitro tissue culture and regeneration. As the newlinemain objective of the study was to genetically transform C4chloroplastic gene to rice cultivar, newlinean efficient tissue culture system establishment was a prerequisite. Based on this fact, newlinestandardisation of tissue culture system was undertaken to establish a proper procedure for newlinecallus mediated transformation. Indica rice genotypes are widely known as recalcitrant in in newlinevitro culture system. In the present study, we evaluated the tissue culture responsiveness of newline20 Indica rice genotypes known for different important characters and standardized the newlinesuitable hormone combination for regeneration of each of those genotypes. ISSR newlinefingerprinting revealed monomorphic banding pattern between regenerated and control newlineplants indicating the suitability of those regeneration media to maintain genetic fidelity. The newlinecall! developed in the study also tested for Agrobacterium mediated genetic transformation newlinestudy. newlineRice operates C3 photosynthetic pathway, a less efficient tha