Development of nanofilms using Catharanthus roseus and Mucuna pruriens for improved lipid oxidative and microbial stability of animal food matrices

Abstract

The silver nanoparticle based bioactive edible films were developed to improve storage stability of animal food matrices. The composite films were developed using carrageenan and hemp seed protein and their bioactive properties were improved using Catharanthus roseus (CaNPs) and Mucuna pruriens (MuNPs) based silver nanoparticles. The preservative potential of the developed films was evaluated using composite meat chocolate, cooked chevon, and cheddar cheese as food models. Three types of product samples were developed viz. T0 (samples within control film), T1 (samples packaged within film containing 2% Catharanthus roseus or 1.5% Mucuna pruriens extract), T2 (samples packaged within T2 film containing 2% CaNPs or 1.5% MuNPs). The films were evaluated for various physico-mechanical parameters and the product samples packaged within the films were evaluated for storage quality, chocolate samples for 180 days, chevon for 14 days, and cheese for 90 days. The addition of the extract or nanoparticles significantly (Plt0.05) increased the mean values for thickness (mm), density (g/ml), redness (a*) and yellowness (b*) whereas decreased the solubility (%), WVTR (mg/mt2), moisture (%), transmittance (%), and lightness (L*) of the films. It also increased (Plt0.05) the total phenolic, total flavonoid and radical scavenging and ion reducing potential (DDPH, ABTS and FRAP) of the films. Application of the films significantly (Plt0.05) improved lipid stability (free fatty acids, thiobarbituric acid reactive substances) and microbial quality (total plate, psychrophilic, yeast/moulds) of the products during storage. A significant (Plt0.05) positive impact of the films was also observed on sensory quality of the products. Based on our results, films T2 containing 1.5% MuNPs or 2% CaNPs significantly (Plt0.05) improved the storage quality of the products compared to the extract based and control films. The developed films can be successfully used commercially for improving storage stability of animal foods which are highly susceptible to lipid oxidation and microbial spoilage. newline