studies on high fat diet induced epigenetic changes in gastrocnemius muscle of type 2 diabetic rats therapeutic role of beta sitosterol

Abstract

2.1 Aim newlineThe aim of this research is to determine whether and#946;-sitosterol has the potential to reverse epigenetic changes that a high-fat diet and sucrose consumption triggered in the rat gastrocnemius muscle newline2.2 The objectives of the study are newline1. To examine the role of and#946;-sitosterol on the expression of genes involved in GLUT4 translocation in the gastrocnemius muscle of rats with type 2 diabetes mellitus (T2DM) induced by a high-fat diet (HFD). 2. To examine the epigenetic alterations occurring in specific genes related to the insulin signalling pathway that display disrupted expression in the gastrocnemius muscle and to find out the influence of and#946;-sitosterol on these epigenetic modifications. newline2.3 Hypothesis newlineBeta sitosterol may have the potential to regulate the translocation of glucose transporters in the GM of adult male rats, thereby mitigating the development of insulin resistance and epigenetic alterations caused by a high fat diet. newline2.4 Institutional animal ethics committee clearance newlineMale Wistar albino rats, or Rattus norvegicus, weighing around 95g and maturing between 150 and 180 days were employed in this investigation. At BRULAC, located at SDC, SIMATS, Chennai 600 077, the animals were treated in compliance with the National Guidelines and Protocols that had been authorised by the Institutional Animal Ethical Committee (IAEC No: BRULAC/SDCH/SIMATS/IAEC/082021/086). They were kept in spotless polypropylene cages, fed a typical rat pelleted diet, and given unlimited access to clean drinking water. newline