In Silico designingof vaccines for genomic targets to human pathogenic bacteria resistance to drugs and development of Genomic target database

Abstract

Bacterial infections, sexually transmitted diseases (STDs), the Gonorrhea caused by N. newlinegonorrhoeae is the second most common STD and a global public health problem due to newlinelack effective drugs and vaccines against the pathogen probably due to the immergence newlineof mutant multi-drug resistance strains. In this research, using novel computational newlineapproaches, several genomic drug and vaccine targets have been identified in N. newlinegonorrhoeae that can be useful to develop next-generation drugs and vaccines against the newlinepathogen. The approach is extended to identify common, conserved, and novel drug and newlinevaccine targets in Corybacterium. Mycobacterium and Nocardia (CMN group) of human newlineand cattle pathogens and one broad spectrum lead compound was identified that can newlinepotentially render multiple targets in theses pathogens. A novel computational approach newline(to design B-celt derived T-cell epitopes that can produce both MHC-I and MHC-I1 newlinemediated immunity) is applied to design best candidate epitopes for N. gonorrhoeae. newlineFinally a Genomic Target Database (GTD) is developed to list putative drug and vaccine newlinetargets of most common human pathogens. This database is a readily available resource newlinein developing drug and vaccine against the respective pathogens. newlineOne review and seven research papers have been derived from this research and the newlineimportant findings of the research are; (1) the N. gonorrhoeae virulent strain FA 1090 newlineconsists of total 537 essential genes that comprises 26% of the pathogen genome and newline29% drug targets of the pathogen are localizes to the membrane. (2) The D-alanine Dalanine newlineligase (ddl). Sulfate transport permease protein C (cysW), and Type IV pilin newlineprotein (pilV) are important targets in pathogen specific unique metabolic pathways and newlineABC transporter (NGO0446) and Competence lipoprotein (ComL) are important targets newlinein host-pathogen common metabolic pathways of N. gonorrhoeae. (3) Other important newlineidentified targets in N. gonorrhoeae genome are: envA, rfaC, tryptophan synthase newlinesubunit a (trpA), indole-3-glycerol-phosph