Development of bio nano composite packaging film for the preservation of food

Abstract

i newlineABSTRACT newlineThe present study aims to produce Bio-nano composite packaging films by utilising the newlineingredients of agriculturally derived products. A novel organic acid-modified starch, and newlinecarboxymethyl cellulose (CMC) based films, reinforced with cellulose nanofiber and plasticized newlinewith glycerol were prepared from tikhur starch (Curcuma angustifolia) by solution casting. Wet newlinemilling was used in the laboratory to extract starch from the tikhur rhizome (Curcuma newlineangustifolia). The low amount of fat, protein, and ash in the extracted starch proved its purity newlineby physical and chemical examination. Functional characteristics indicated its potential for newlinebiodegradable film development. Carboxymethyl cellulose (0.2 gand#8722;1 newlinestarch dry basis) was blended newlinewith starch to improve its film-forming properties. Cellulose nanofiber (CNF) from banana plant newlinewaste (pseudo-stems) was produced via combined enzymatic hydrolysis and chemical treatment. newlineThe XRD analysis revealed a high degree of crystallinity (60.13%) with enzymatic hydrolysis newline(70 units/gram of fiber) followed by mild acid treatment (5% oxalic acid) of CNFs. After newlinecomparing different methods for producing cellulose nanofiber, it was found that alkali newlinepretreatment at room temperature for a longer duration (25 and#8451; for 14 h) leads to high crystallinity newlinewith a more symmetric crystal structure as compared to alkali treatment at a higher temperature newlinefor a short duration under steam (121 and#8451; for 1 hr. in an autoclave). The produced CNF from newlinebanana pseudo-stems was utilized as a reinforcement agent in the starch/CMC-based film. Three newlinedifferent treatments with varying organic acids (lactic acid, citric acid, and acetic acid) at a newlineconcentration of 5% w/w of starch (2ppm) in a film-forming solution were done. The effect of newlineorganic acid incorporation on the antimicrobial, morphological, structural, thermal, and newlinecrystalline properties of developed films was studied. The Minimum Inhibitory Concentration newline(MIC) values of the three organic acids against gram-negative (E.coli)