Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/5043
Full metadata record
DC FieldValueLanguage
dc.coverage.spatial--en_US
dc.date.accessioned2012-11-05T06:40:45Z-
dc.date.available2012-11-05T06:40:45Z-
dc.date.issued2012-11-05-
dc.identifier.urihttp://hdl.handle.net/10603/5043-
dc.description.abstractOpportunistic infections in human immunodeficiency virus (HIV) infected patients are often associated with an increase in HIV replication. Due to the high incidence of both HIV and Mycobacterium sp. infection in the developing countries, tuberculosis (TB) has emerged to be the most common opportunistic infection in HIV-infected patients world-wide. Co-infection with the bacilli has been postulated to be an important exogenous factor that influences the severity and rate of disease progression in HIV-infected individuals, reducing the chances of survival and increasing the risk of HIV transmission. Macrophages act as a host to both M. tuberculosis and HIV and hence appear to be a possible site of interaction for these two deadly pathogens. The incidence of HIV type 1 (HIV-1) infection is found to be accelerated in people infected with Mycobacterium tuberculosis. However, the mechanisms by which mycobacterial protein(s) enhance transcriptional activity of HIV-1 LTR, the sole promoter element of HIV (Bernier et al., 1998, Kitaura et al., 2001), is not clearly understood. In the present study it has been demonstrated that one of the M. tuberculosis PPE proteins, Rv1168c can trans-activate HIV-1 LTR in monocyte/macrophage cells by targeting the NF-and#954;B signaling cascades. The NF-and#954;B is one of the most important factors involved in the transcription of HIV-1 LTR (Nabel et al., 1987, Asin et al., 2001, Rothwarf et al., 1999). Western blotting and EMSA results indicate that Rv1168c up-regulates p50 and p65 protein expression and its DNA-binding activity. The Rv1168c-mediated increase in HIV-1 LTR activity was found to be predominantly dependent on NF-and#61547;B transcription factor as inhibition of NF-and#61547;B signaling by either treating cells with the specific pharmacological inhibitor, PDTC or by over-expressing phosphorylation defective Iand#61547;Band#945; decrease transcription from the HIV-1 LTR promoter by more than 90% in cells treated with Rv1168c.en_US
dc.format.extent247p.en_US
dc.languageEnglishen_US
dc.relation--en_US
dc.rightsuniversityen_US
dc.titleRegulatory role of Mycobacterium tuberculosis PPE proteins on proinflammatory signaling pathway and activation of HIV-1 LTRen_US
dc.creator.researcherBhat, Khalid Hussainen_US
dc.subject.keywordHuman Immunodeficiency Virusen_US
dc.subject.keywordDNAen_US
dc.subject.keywordTLR2en_US
dc.subject.keywordHIV-1 LTRen_US
dc.subject.keywordCoinfectionen_US
dc.subject.keywordMycobaterium tuberculosisen_US
dc.subject.keywordRv0256cen_US
dc.subject.keywordRv1168cen_US
dc.subject.keywordPE proteinsen_US
dc.subject.keywordPPE proteinsen_US
dc.description.noteBibliography p. 214-247, Appendix includeden_US
dc.contributor.guideMukhopadhyay, Sangitaen_US
dc.publisher.placeManipalen_US
dc.publisher.universityManipal Universityen_US
dc.publisher.institutionCentre for DNA Fingerprinting and Diagnostics, Hyderabaden_US
dc.date.registered23/06/2006en_US
dc.date.completed22/12/2011en_US
dc.date.awarded27/04/2012en_US
dc.format.dimensions--en_US
dc.format.accompanyingmaterialNoneen_US
dc.type.degreePh.D.en_US
dc.source.inflibnetINFLIBNETen_US
Appears in Departments:Centre for DNA Fingerprinting and Diagnostics, Hyderabad

Files in This Item:
File Description SizeFormat 
01_title.pdfAttached File197.47 kBAdobe PDFView/Open
02_certificate.pdf887.58 kBAdobe PDFView/Open
03_dedcation.pdf28.56 kBAdobe PDFView/Open
04_declaration.pdf.pdf840.17 kBAdobe PDFView/Open
05_abstract.pdf173.29 kBAdobe PDFView/Open
06_acknowledgement.pdf36.92 kBAdobe PDFView/Open
07_list of table.pdf30.23 kBAdobe PDFView/Open
08_list of figures.pdf75.68 kBAdobe PDFView/Open
09_contents.pdf205.28 kBAdobe PDFView/Open
10_abbreviations.pdf158.7 kBAdobe PDFView/Open
11_chapter 1.pdf2.42 MBAdobe PDFView/Open
12_chapter 2.pdf2.01 MBAdobe PDFView/Open
13_chapter 3.pdf2.12 MBAdobe PDFView/Open
14_chapter 4.pdf219.36 kBAdobe PDFView/Open
15_bibliography.pdf319.08 kBAdobe PDFView/Open
16_appendix.pdf205.47 kBAdobe PDFView/Open


Items in Shodhganga are licensed under Creative Commons Licence Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0).

Altmetric Badge: