Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/500863
Full metadata record
DC FieldValueLanguage
dc.coverage.spatial
dc.date.accessioned2023-07-21T09:45:30Z-
dc.date.available2023-07-21T09:45:30Z-
dc.identifier.urihttp://hdl.handle.net/10603/500863-
dc.description.abstractTransfusion of healthy RBCs is a lifesaving process. However, upon storing Non-Leukoreduced (NLR) and Leuko reduced (LR) RBCs, a wide range of Damage Associated Molecular Patterns (DAMPs), such as cell-free DNA, nucleosomes, free-haemoglobin, and poly-unsaturated-fatty-acids (PUFAs), are generated. These DAMPs can further damage RBCs. Thus, the quality of stored RBCs declines during storage and limits their shelf life. Transfusion of old stored whole blood consists of a pool of DAMPs are known to be primary mediators in the pathophysiology of fatal diseases like Transfusion-Related Acute Lung Injury (TRALI), deep-vein thrombosis, and organ dysfunction. Since these DAMPs contain either positive or negatively charged species. Since these DAMPs consist of either positive or negative charged species, we developed taurine and acridine-containing Electrospun-nanofibrous-sheets (Tau-AcrNFS), featuring anionic, cationic charges and a DNA intercalating group on their surfaces. We show that Tau-AcrNFS efficiently scavenges DAMPs from stored human and mouse RBCs ex vivo. We find that intermittent scavenging of DAMPs by Tau-AcrNFS during the storage reduces the loss of RBC membrane integrity and reduces discocytes-to-spheroechinocytes transformation in stored old RBCs. We perform RBC-transfusion studies in mice to reveal that intermittent removal of DAMPs enhances the quality of stored old RBCs equivalent to freshly collected RBCs and increases their shelf-life by ~22%. Additionally, we have demonstrated that treating stored old blood with nanofibrous sheets prior to transfusion can prevent TRALI, in vivo newline
dc.format.extent
dc.languageEnglish
dc.relation
dc.rightsself
dc.titleDeveloping Nanofibrous Sheets to Scavenge Storage Lesion to Enhance the Quality and Shelf Life of Stored Red Blood Cells and Prevent Transfusion related Complications
dc.title.alternative
dc.creator.researcherPandey, Subhashini
dc.subject.keywordClinical Medicine
dc.subject.keywordClinical Pre Clinical and Health
dc.subject.keywordDAMPs
dc.subject.keywordDiscocytes
dc.subject.keywordElectrospun-NFS
dc.subject.keywordLeukoreduced
dc.subject.keywordNon-Leukoreduced
dc.subject.keywordPathology
dc.subject.keywordRBCs
dc.subject.keywordTransfusion
dc.description.note
dc.contributor.guideVemula, Praveen Kumar
dc.publisher.placeBangalore
dc.publisher.universityInstitute of Trans-disciplinary Health Science and Technology
dc.publisher.institutionCentre for Clinical Research and Education
dc.date.registered
dc.date.completed2023
dc.date.awarded2023
dc.format.dimensions
dc.format.accompanyingmaterialNone
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:Centre for Clinical Research and Education

Files in This Item:
File Description SizeFormat 
01-title page.pdfAttached File265.67 kBAdobe PDFView/Open
02-prelim pages.pdf780.06 kBAdobe PDFView/Open
03-content.pdf288.43 kBAdobe PDFView/Open
04-abstract.pdf115.04 kBAdobe PDFView/Open
05-chapter-1.pdf475.79 kBAdobe PDFView/Open
06-chapter-2.pdf2.21 MBAdobe PDFView/Open
07-chapter-3.pdf1.17 MBAdobe PDFView/Open
08-chapter-4.pdf549.76 kBAdobe PDFView/Open
09-annexure.pdf525.12 kBAdobe PDFView/Open
80_recommendation.pdf297.33 kBAdobe PDFView/Open


Items in Shodhganga are licensed under Creative Commons Licence Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0).

Altmetric Badge: