In vitro anti cancer activity of Limonia acidissima extract on breast cancer cell MCF 7

Abstract

In the present study the crude extract of Limonia acidissima roots, fruits, leaves and stem bark were investigated for its important phytochemical properties and observed the Anti-cancer activity of Limonia acidissima against breast cancer MCF-7 cell line. Limonia acidissima belongs to family Rutaceae. In today s the researchers are emphasizing on evaluation and characterization of various plants constituents and such plant constituents used against to treat diseases based on their traditional claims given in Ayurveda. Phytochemical screening was done using standard methods. Extraction of the bioactive plant constituents was done by solvent extraction method with various solvents and purification was done by using TLC and column chromatography. Moreover, IR, NMR, MS was done for the structural analysis of recoverd anti-cancerous metabolites from methanolic crude extract (fruit) of Limonia acidisima. Ethanol, methanol, chloroform and Aqueous extract of leaves, roots, fruits and stembark was tested for the presence and absence of Alkaloids, Terpenoids, Saponins, Stilbene, Carbohydrates and Glycosides, Quinone, Anthraquinones, Steroids and Phytosteroids, Flavonoids, Cardiac glycosides, phenolics compounds and Tannins, Proteins, Amino acids and Phlobatannins. The result of the study showed Different crude extract of Limonia acidissima at concentrations 50, 100, 200 and 400µg/ml were incubated with MCF-7 for 48 hrs. Cell viability was evaluated by the reduction of the yellow colour MTT to the purple formazan. Most of the crude extracts (leaves, roots, and stem bark) were found to be showed lowest toxicity to cancer cell line. However, the methanol extracts of fruits exhibit good cytotoxic activity 59±1.0, 33.3±1.2, 29.8±0.9 and 26.3±0.6 at a concentration of 50,100,200 and 400 (and#956;g/mL) respectively. The methanolic fruits extract showed higher percentage of cell viability 63.61 at 12.25 µg/ml and lowest percentage cell viability 21.32 at 200 µg/ml. IC50 concentration of methanol extract of fruits IC50: 55.26±1.41µg/ml.