Pharmacological And Toxicological Evaluation Of Polyherbal Formulations In Hyperglycemia

Abstract

newline As per (WHO), 70~80 % of the people across globe have conventional remedies as part of their health management regime (Gao,2011). Since ancient times herbal sources are used as medicine (Ibrahim et al,2016). newlineThe purpose of the current study is to prepare a polyherbal formulation whose formula is given in Ayurvedic Formulary of India and to evaluate its anti-hyperglycaemic potential by In-Vitro and In-Vivo methods supported by toxicological profile establishment by using OECD guidelines 423 and 407 respectively. Avipattikara Churna (as Formulation-1) and Triphala Churna (as Formulation-2) were selected for the study after exhaustive literature survey. During the research study we have assessed various parameters to provide well-established documented evidence. The research was started by procurement of ingredient plants from an authentic source of both formulations(F1andF2). Initially, the plant materials were authenticated, then shade dried and grinded to course powder for extraction purposes after mixing as per the formula given in AFI. Two extracts were taken in the studies-Aqueous and Methanolic. After primary pharmacognostical investigations as per the WHO guidelines of herbal standardization, we have moved towards biological assessments. Acute toxicity studies were performed as per guidelines of OECD 423 for dose selection for all extracts in both the formulations. After that In-vitro alpha-Amylase and alpha-Glucosidase inhibitory assay were performed for both the formulations. In-Vivo antidiabetic studies were done in STZ + Nicotinamide induced type -II diabetes in Wistar rats for F1 and F2 at two different doses for Aqueous and Methanolic extracts. The whole dosing schedule was divided into 11 groups including control group. Various parameters like-body weight, food intake, Oral Glucose Tolerance, lipid profile and histopathological studies of pancreas, liver and kidney were performed during the In-vivo studies. As per the results obtained one best formulation (F1) were selected for performin