Development of Standardization Parameters and Analytical Profiling of Moringa Species of India

Abstract

The variety of species of moringa genus were cultivated globally; two species out of 13 species of Moringa are cultivated in india viz., Moringa oleifera and Moringa concanensis. Moringa concanensis Nimmo (Moringaceae) is tropically distributed in deciduous forests of India. The plant has remarkable nutritional value along with impressive medicinal uses. The standardization parameters are required for quality assessment of plant material. The current investigation reveals determinations related to pharmacognostic and phytochemical parameters of M. concanensis leaves. Due to the bitter taste of plant it is not edible. These investigations are helpful to develop the formulations including Moringa concanensis as individual or in combination. newlineTribes used this plant traditionally as an; antioxidant, anticancer, anticonvulsant, antimicrobial, antifertility, anti-inflammatory, antipyretic and analgesic. In current study, Moringa concanensis leaves were subjected to Soxhlet extraction which were sequentially extracted with five different solvents of increasing polarity i.e., petroleum ether, chloroform, ethyl newlineacetate, methanol and water. After phytochemical investigations indicate the presence of steroids, flavonoids, cardiac glycosides, terpenoids, alkaloids, carbohydrates, proteins, phenols and tannins. The chloroform extract reveals the existence of cardiac glycosides (cardenolides) newlinetherefore further RP-HPLC method were progressed to identify the number of constituents present in extract. The extract was recrystallized to isolate the single component and to gain the desired purity for further spectral analysis. Different spectral data from UV-Visible, Mass, Infrared, and Nuclear Magnetic Resonance (NMR) spectroscopy were analyzed and the phytoconstituent was identified. The current investigation, elaborates reverse-phase high-performance liquid chromatography (RP-HPLC) method development and validation to quantify digoxin in chloroform extract of newlineM. concanensis leaves from three different geographical sources.