Method development and validation for metal analysis in natural marketed products using inductive coupled plasma optical emission spectroscopy

Abstract

viii newlineABSTRACT newlineHerbal medicines have been using for the treatment, management, and prevention newlineof different types of diseases by majority of the population in India since time newlineimmemorial. These medicines are effective besides being easily available, and newlineinexpensive compared with allopathic drugs. In recent times, several cases of heavy newlinemetals in herbal medicine have come to light. As a result, regulators have started newlinemonitoring for controlling the quality of herbal medicines. The present study newlinepertains to the development of a validated method to determine the content of lead, newlinecadmium, arsenic, and mercury in different herbs e.g. Cardorium plus, newlineAbhrakBhasma and Saraswatarishta. Since these herbs are widely used in herbal newlinemedicines, the method would be of high value. Inductive coupled plasma optical newlineemission spectroscopy was used for this purpose. The quality and safety of herbal newlineformulations has become serious issue, as this Indian medicine is used by 80% of newlinethe Indian population. Hence, the present study was performed to evaluate heavy newlinemetals contents by Inductive coupled plasma optical emission spectroscopy. newlineFirst, instrumental parameters were optimized and then analytical lines free from newlinespectral interferences were selected. The proposed method showed good linearity newlinebetween the peak area of each analyte and its concentration (r2and#8805;0.9991). The newline%RSD for system precision for arsenic, lead, cadmium and mercury for Cardorium newlineplus were found to be 5.98, 1.08, 4.92 and 2.76 respectively. The percentage newlinerecoveries for 100% spike sample for method precision for Cardorium plus were newlinewithin the 88.3-107.5% range. The % recovery for various preparations for 50% newlinespike sample of Cardorium plus was in the range of 90 to 110%. The % recovery for newlinevarious preparations for 100% spike sample of Cardorium plus was in the range of newline95 to 106.7%. The % recovery for various preparations for 150% spike sample of newlineix newlineCardorium plus was in the range of 93.3 to 103.3%. Ruggedness of the proposed newlinemethod was determined and the obtained spike rec