Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/485449
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dc.coverage.spatialMolecular Biology
dc.date.accessioned2023-05-24T11:33:59Z-
dc.date.available2023-05-24T11:33:59Z-
dc.identifier.urihttp://hdl.handle.net/10603/485449-
dc.description.abstractThe aim was to characterize the genome mined putative epoxide hydrolase gene (sgeh) from Streprtomyces griseus. The purified overexpressed recombinant SGEH enzyme was enantioselective and had broad substrate spectrum. The reaction parameters of the recombinant culture were optimized to kinetically resolve phenyl glycidyl ether into its R-enantiomer with the E-value of 20. The study led to an eco-friendly process to produce 98% enatiopure (R)-PGE for the production of chiral amino alcohols and Beta- blockers. newline
dc.format.extentxiv, 201p.
dc.languageEnglish
dc.relation-
dc.rightsuniversity
dc.titleCloning and Characerizaion of epoxide hydrolase from Streptomyces griseus for bioresolution of epoxides
dc.title.alternative
dc.creator.researcherSaini, Priya
dc.subject.keywordBioresolution
dc.subject.keywordEpoxide hydrolase
dc.subject.keywordPhenyl glycidyl ether
dc.subject.keywordRecombinant protein
dc.subject.keywordStreptomyces griseus NBRC 13350
dc.description.noteBibliography 166-195p. Annexure 196-201p.
dc.contributor.guideSareen, Dipti
dc.publisher.placeChandigarh
dc.publisher.universityPanjab University
dc.publisher.institutionDepartment of Biochemistry
dc.date.registered2011
dc.date.completed2017
dc.date.awarded2017
dc.format.dimensions-
dc.format.accompanyingmaterialCD
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:Department of Biochemistry

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01_title.pdfAttached File6.49 kBAdobe PDFView/Open
02_prelim pages.pdf1.15 MBAdobe PDFView/Open
03_chapter1.pdf367.39 kBAdobe PDFView/Open
04_ chapter2.pdf143.58 kBAdobe PDFView/Open
05_chapter3.pdf1.92 MBAdobe PDFView/Open
06_chapter4.pdf1.6 MBAdobe PDFView/Open
07_chapter5.pdf6.46 MBAdobe PDFView/Open
08_chapter6.pdf394.31 kBAdobe PDFView/Open
09_annexures.pdf1.04 MBAdobe PDFView/Open
80_recommendation.pdf198.42 kBAdobe PDFView/Open


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