Genetic engineering of suicide genes to improve therapeutic efficacies

Abstract

Gene Directed Enzyme Prodrug Therapy also known as Suicide gene therapy SGT has the potential to provide specific targeting of the tumor cells thereby conquer the deteriorate effects of chemotherapy treatment of cancer As the inefficient binding of the prodrug s at the active site of the key enzymes which are involved in the SGT applications the present thesis focused mainly on the development of novel suicide gene mutants to boost up their therapeutic efficacies Bacterial expression vect